大鼠“血瘀证”下急性心肌梗死模型的建立与评价

发布时间：2018-04-18 05:10

本文选题：血瘀证 + 急性心肌梗死　；参考：《吉林大学》2007年硕士论文

【摘要】： 目前的中药药理研究,绝大多数动物实验还是采用西医病因病理复制动物模型,只强调“病”,而忽略了“证”,很难通过动物模型反映中医“辨证施治”的治疗原则和中药的治疗特点。如何将中医征候与疾病有机结合起来,建立“病”和“证”相统一的实验动物模型,使之具有可重复性和操作性,是中药药理研究的一个关键技术平台。以往在研究治疗胸痹心痛(冠心病心绞痛)的中药时,通常选用西医的大鼠急性心肌梗死病理模型进行评价,忽视了中医的征候。而临床上的胸痹心痛可见多种中医“证侯”,如“血瘀证”。为确证单纯病理模型能否准确反映出不同的中医“证侯”,我们建立了大鼠“血瘀证”下急性心肌梗死病理模型,通过NB-T染色计算梗死范围、心肌三酶、心电变化、自由基及抗氧化酶、内皮损伤相关物质、心肌代谢物质、“血瘀证”特征因子、血小板粘附性及聚集性、体外血栓形成、红细胞压积及血沉及血流动力学等多种指标与单纯心肌梗死的病理模型进行比较,同时采用中药和西药进行药物干预。结果表明,尽管“血瘀证”下急性心肌梗死模型在心肌形态学、血液生化学、血液流变学、血流动力学等各方面比“单纯”急性心肌梗死模型的变化程度增大,但两者之间无明显差异,而且与西药和中药的干预作用结果相一致。提示大鼠单纯心肌梗死模型可以用来评价血瘀证下心肌梗死,无须建立“急性心肌梗死”和“血瘀证”并存的实验动物模型。但单纯西医的病理模型是否可以代替中医“证”与“病”并存的征候模型,尚无前人报道。
[Abstract]:In the current pharmacological study of traditional Chinese medicine, the vast majority of animal experiments still use Western medicine to make animal models of etiology and pathology, emphasizing only "disease" and neglecting "syndrome".It is difficult to reflect the treatment principle of TCM and the characteristics of TCM by animal model.How to combine TCM symptoms with diseases organically, and establish a unified experimental animal model of "disease" and "syndrome", so as to make it repeatable and operable, is a key technical platform for the pharmacological research of traditional Chinese medicine (TCM).In the past, the traditional Chinese medicine for the treatment of chest arthralgia and angina pectoris was usually evaluated by the pathological model of acute myocardial infarction in rats with western medicine, and the symptoms of TCM were ignored.Clinical chest pain can be seen in a variety of traditional Chinese medicine, such as "blood stasis syndrome."In order to confirm whether the simple pathological model can accurately reflect different TCM "syndromes", we established a pathological model of acute myocardial infarction under "blood stasis syndrome" in rats. The infarct size, myocardial trienzyme and ECG changes were calculated by NB-T staining.Free radical and antioxidase, endothelium-related substances, myocardial metabolites, characteristic factors of "blood stasis syndrome", platelet adhesion and aggregation, thrombus formation in vitro,The hematocrit, erythrocyte sedimentation rate and hemodynamics were compared with the pathological model of myocardial infarction.The results showed that the changes of myocardial morphology, blood biochemistry, hemorheology and hemodynamics in acute myocardial infarction model under "blood stasis syndrome" were larger than those in "pure" acute myocardial infarction model.But there was no significant difference between the two, and the results were consistent with the intervention of western medicine and traditional Chinese medicine.It is suggested that the simple myocardial infarction model can be used to evaluate myocardial infarction under blood stasis syndrome, and it is not necessary to establish an experimental animal model of "acute myocardial infarction" and "blood stasis syndrome".But whether the pathological model of western medicine can replace the symptom model of "syndrome" and "disease" in traditional Chinese medicine has not been reported before.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R-332

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