抗脊髓灰质炎病毒Ⅱ型Sabin株D抗原单克隆抗体的制备及其在Sabin IPV效力检测中的应用

发布时间：2018-04-18 04:26

  本文选题：脊髓灰质炎病毒 + Sabin株　； 参考：《昆明医学院》2006年硕士论文

【摘要】：脊髓灰质炎(poliomyelitis)(脊灰)是一种传播广泛、侵犯中枢神经系统的病毒性传染病，自1988年实施消灭脊髓灰质炎(脊灰)战略以来，我国通过接种口服脊髓灰质炎减毒活疫苗(OPV)消灭了本土野毒株引起的脊髓灰质炎。但是仍存在OPV引起的疫苗相关麻痹病例(VAPP)和疫苗衍生株(VDPV)，脊髓灰质炎灭活疫苗(IPV)基本可避免OPV上述缺点。WHO指出当全球证实消除脊灰后，，将停止使用OPV，届时可能需要脊髓灰质炎灭活疫苗(IPV)来长期保持脊灰免疫水平。相对于野毒株IPV，Sabin株IPV在制造和使用过程中更安全，对它的研究受到了WHO的高度重视。目前生产减毒株灭活疫苗的国家有中国、日本及荷兰。我所正在积极研制Sabin IPV，D抗原量是体外测定IPV效力的关键指标，与疫苗在人体内免疫效力有较好的一致性。目前国际上尚缺乏Sabin IPV D抗原检测的标准化的试剂，本实验应用杂交瘤技术所制备的抗Ⅱ型Sabin株D抗原单克隆抗体分子量为150KD；纯化抗体的ELISA效价为1：2.4×10~6，中和抗体效价为1：1.3×10~4；与Ⅱ型Sabin株C抗原、Ⅰ型、Ⅲ型Sabin病毒抗原不发生交叉反应，特异性良好。并用此单克隆抗体的ELISA法与本室建立的多抗方法进行比较，同时对三价Sabin IPV疫苗中Ⅱ型D抗原的含量进行测量，发现在0.125Du／ml-2 Du／ml范围内应用单抗测量D抗原的直线相关系数为0.998、0.966、0.903；而使用本室建立的多抗方法直线相关系数为1.000、0.998、0.986。本实验制备的单克隆抗体具有良好的特异性，可为Sabin IPV体外效力检测的标准化奠定基础。
[Abstract]:Poliomytis poliomyelitis (poliomyelitis) is a viral infectious disease that is widely spread and invades the central nervous system. Since the implementation of the poliomyelitis (polio) eradication strategy in 1988,Our country eliminated poliomyelitis caused by native wild strain by inoculating oral live attenuated poliomyelitis vaccine (OPVV).But there are still vaccine-related paralysis cases caused by OPV) and the vaccine derived strain, poliomyelitis inactivated vaccine, which can basically avoid the above-mentioned disadvantages of OPV. Who points out that when polio is confirmed to be eliminated globally,OPVs will be discontinued, when polio inactivated vaccine IPV may be required to maintain polio immunization over the long term.Compared with the wild strain IPV Sabin strain IPV is safer in manufacture and use, and its research has been paid more attention to by WHO.Currently, China, Japan and the Netherlands are producing attenuated inactivated vaccines.Our institute is actively developing Sabin IPV D antigen, which is the key index to determine the potency of IPV in vitro, and is in good agreement with the immune efficacy of the vaccine in human body.At present, there is a lack of standardized reagent for the detection of Sabin IPV D antigen in the world.The molecular weight of monoclonal antibody against D antigen of type 鈪

本文编号：1766801