PRP诱导人BMSc体内成骨及免疫配型不相合人BMSc混合培养实验研究

发布时间：2018-04-19 06:11

本文选题：骨 + 组织工程　；参考：《第一军医大学》2006年博士论文

【摘要】：由创伤、感染、肿瘤等疾病引起的骨缺损是骨科临床的常见病，骨缺损治疗的难题主要是移植骨的来源问题。组织工程的兴起和发展为骨缺损的修复开辟了一条新的途径。骨组织工程技术以体外构建、体内重组的模式给这一领域提供了崭新的治疗技术手段，成为目前修复骨缺损的较为理想的方法。随着研究的深入，骨组织工程已逐步由动物实验扩展到临床实验阶段。临床应用不同于动物实验，首先需要考虑治疗的安全性，因此组织工程种子细胞的选择，材料的选择以及种子细胞的体外培养过程等问题都是需要谨慎注意的。富血小板血浆是经过特殊方法提取的血小板含量丰富的血浆，相较普通血清含有更丰富的细胞因子。研究表明细胞因子是骨髓基质干细胞增值、分化的必须。本研究以自体骨髓基质干细胞作为种子细胞，尝试采用自体富血小板血浆替代普通血清——尽量排除异类抗原物质的引入且利于促进细胞的增值、分化的培养方法，诱导培养人骨髓基质干细胞，并将诱导培养的骨髓基质干细胞与临床应用相对成熟具有临床应用许可的北京意华健公司的YHJ500珊瑚羟基磷灰石材料复合植入裸鼠体内。探讨了自体富血小板血浆对诱导培养人骨髓基质干细胞生物学特性的影响及以自体富血小板血浆诱导培养的人骨髓基质干细胞与珊瑚羟基磷灰石材料的生物相容性及在体内的成骨效用。由于自体骨髓基质干细胞培养、扩增需很长时间且针对年龄较大或存在其他疾患的个体又无法完成自体的骨髓基质干细胞的培养与扩增，因此建立人骨髓基质干细胞库是组织工程在临床推广应用的必须。研究证实异体骨髓基质干细
[Abstract]:By trauma, infection, tumor and other diseases caused by bone defect is a common disease in clinical department of orthopedics, problems in the treatment of bone defect is the main source of bone graft. The rise and development of tissue engineering provides a new way for the repair of bone defect. Bone tissue engineering technique to construct the recombinant in vitro, in vivo model provide a new treatment technology for this field, becoming the bone defect repair is an ideal method.
With the in-depth study, bone tissue engineering has been gradually extended from animal experiments to clinical trials. The clinical application is different from the animal experiments, first of all need to consider the safety of the treatment, the seed cells of tissue engineering, material selection and seed cells in vitro culture process is need careful attention. Platelet rich the plasma is extracted by special method in platelet rich plasma, compared with common serum contained more rich cytokine. The study showed that the cell factor is bone marrow stromal stem cell differentiation. This value must be studied with autologous bone marrow stromal stem cells as seed cells, try using autologous platelet rich plasma instead of normal serum -- try to exclude alien the introduction of an antigenic substance promote cell proliferation and differentiation, cultivation method, induced culture of human bone marrow stromal stem cells, and induced YHJ500 coral hydroxyapatite composite implantation of bone marrow stromal stem cell culture and clinical application of the relatively mature clinical application has permitted Beijing Huajian company. Discusses the compatibility and in vivo osteogenic effect of autologous platelet rich plasma effects on the biological characteristics of cultured human bone marrow stromal stem cells and autologous platelet rich plasma cultured human bone marrow stromal stem cells and coralline hydroxyapatite biological materials.
Because of autologous bone marrow stromal stem cell culture, expansion and take a long time for older or have other disorders of the individual is unable to complete the cell culture and amplification of bone marrow stromal stem autologous, so the establishment of human bone marrow stromal stem cell bank is tissue engineering in clinical application. It must be confirmed that allogeneic bone marrow stromal stem cells

【学位授予单位】：第一军医大学
【学位级别】：博士
【学位授予年份】：2006
【分类号】：R329.2

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