快速大容量尾静脉注射法构建循环肝癌细胞肝转移小鼠模型

发布时间：2018-04-20 20:08

本文选题：循环肝癌细胞 + Hepa-细胞　；参考：《中国肿瘤生物治疗杂志》2014年05期

【摘要】：目的:通过快速大容量尾静脉注射方法构建循环肝癌细胞肝内复发转移小鼠模型。方法:40只C57BL/6J小鼠采用随机数字表法随机分为对照组和实验组(20只/组),对照组采用传统的缓慢小容量尾静脉注射法(2×106个肝癌Hepa1-6细胞/0.2 ml,30 s内注射完毕),实验组采用改良的快速大容量尾静脉注射法(2×106个肝癌Hepa1-6细胞/2 ml,5 s内注射完毕)构建循环肝癌细胞肝内复发转移小鼠模型。4周后摘取肝、肺、肾、脾组织并行H-E染色,大体和镜下观察肝脏、肺脏、脾脏和肾脏的成瘤情况。结果:对照组小鼠只在肺脏有转移灶形成,成瘤率为95%(19/20),肝脏、肾脏、脾脏未见有转移灶形成。实验组小鼠在肝脏和肺脏同时形成转移灶,肺脏成瘤率为94.7%(18/19),肝脏成瘤率为100%(19/19),脾脏、肾脏未见转移灶形成。结论:快速大容量尾静脉注射法注射循环肝癌细胞构建的小鼠模型可以模拟肝癌根治性切除后残留的循环肝癌细胞导致早期肝癌肝内复发转移的过程。
[Abstract]:Objective: to establish a mouse model of intrahepatic recurrence and metastasis of circulating liver cancer cells by rapid large volume caudal vein injection. Methods 40 C57BL/6J mice were randomly divided into two groups: control group (n = 20) and experimental group (n = 20). The control group (n = 20) was injected with 2 脳 10 6 Hepa1-6 cells per 0.2 ml in 30 s. The mice model of hepatic recurrence and metastasis of circulating hepatoma cells was established by a modified rapid large volume caudal vein injection method of 2 脳 106 hepatoma Hepa1-6 cells / 2 ml / min within 5 seconds. Lung, kidney and spleen were stained with H-E staining. Liver, lung, spleen and kidney were observed under microscope. Results: in the control group, metastasis was only found in the lung, and the tumorigenesis rate was 95% 20%. No metastasis was found in the liver, kidney and spleen. In the experimental group, the metastatic foci were formed in the liver and lung simultaneously, the tumorigenesis rate of the lung was 94.770 / 19, the tumorigenesis rate of the liver was 100 / 19 / 19, the spleen and the kidney were not metastasized. Conclusion: the mouse model established by rapid large volume caudal injection of circulating hepatoma cells can simulate the process of recurrence and metastasis of early liver cancer caused by residual circulating hepatoma cells after radical resection of liver cancer.
【作者单位】：苏州大学医学部临床医学院;第二军医大学东方肝胆外科医院分子肿瘤研究室;
【基金】：国家传染病重大专项课题资助项目(No.2012ZX10002012-10) 国家自然科学基金资助项目(No.81272669,No.81301830)~~
【分类号】：R735.7;R-332

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