免疫正常人群和免疫异常人群血清中类胰蛋白酶含量的测定

发布时间：2018-04-21 19:51

本文选题：类胰蛋白酶 + 过敏性猝死　；参考：《山西医科大学》2007年硕士论文

【摘要】： 目的:通过测定免疫正常人群和免疫异常人群血清中类胰蛋白酶的含量,来建立中国免疫正常人群其血清含量的正常值范围,为过敏性疾病和过敏性猝死提供客观、准确的依据。方法:免疫正常人群按年龄分为四组,第一组小于21岁,第二组为21到40岁,第三组41到60岁,第四组大于60岁。免疫异常人群按疾病分为五组,分别为哮喘组、湿疹组、肾炎组、类风湿关节炎组、肝硬化组。收集不同死因的尸体心血4例。使用UniCAP 100过敏原自动检测仪,利用荧光免疫酶技术(FEIA)测定其血清中类胰蛋白酶的含量。结果:(1)正常人血清中tryptase含量的几何均数为2.69μg/L,95%分位数为6.00μg/L。正常人血清中tryptase的含量,男性均值为3.33±1.63μg/L,女性均值为2.80±1.40μg/L,男女之间的差别无统计学意义;四个年龄组组间比较:第1、2、3组均值分别为2.56±1.44μg/L、2.90±1.32μg/L、2.92±1.17μg/L,第4组均值为3.87±2.04μg/L,和1、2、3组相比均有明显差异,比前三个组的均值高。(2)哮喘组均值为6.14±0.35μg/L,湿疹组均值为2.22±1.73μg/L,肾炎组均值为0.80±0.21μg/L,类风湿关节炎组均值为10.42±12.52μg/L,肝硬化组均值为3.13±1.73g/L,正常人组均值为3.06±1.53μg/L。哮喘和类风湿关节炎病人的均值比正常人的均值高;湿疹、肾炎和肝硬化病人的均值与正常人相比,无明显差异。(3)4例尸体心血中类胰蛋白酶的含量分别为24.00μg/L、6.71μg/L、4.83μg/L、19.7μg/L。结论:(1)中国免疫正常人群血清中类胰蛋白酶的含量,几何均数为2.69μg/L,正常范围为[0,6.00]μg/L。其含量无性别差异,但年龄因素对其有一定影响。(2)哮喘和类风湿关节炎病人血清中类胰蛋白酶的含量要高于正常人,湿疹、肾炎和肝硬化病人血清中类胰蛋白酶的含量与正常人相比无明显差异。(3)吸毒和外伤致死的尸体血清中类胰蛋白酶的含量要远远高于正常人,而动脉粥样硬化致死的尸体其含量则无明显变化。
[Abstract]:Objective: to establish the normal range of serum content of Chinese immune normal population by measuring the content of trypsin like trypsin in serum of normal immune people and immune abnormal crowd, to provide objective and accurate basis for allergic diseases and sudden sudden death. Methods: the normal immune population was divided into four groups according to age, the first group was less than 21 years old and second. The group was 21 to 40 years old, third groups 41 to 60 years old and fourth groups more than 60 years old. The immune abnormal people were divided into five groups according to the disease. They were asthma group, eczema group, nephritis group, rheumatoid arthritis group, liver cirrhosis group. The body blood of different causes of death was collected in 4 cases. The UniCAP 100 allergen automatic test instrument was used and the serum FEIA was used to determine its serum. Results: (1) the geometric mean of tryptase content in normal human serum was 2.69 mu g/L, and the number of 95% quantiles was 6 mu g/L. in normal human serum, the mean value of male was 3.33 + 1.63 g/L, the mean value of women was 2.80 + 1.40 u g/L, and there was no statistical difference between men and women; four age groups were compared: 1,2,3 The mean value of the group was 2.56 + 1.44 g/L, 2.90 + 1.32 mu g/L and 2.92 + 1.17 u g/L, and the mean value of the fourth group was 3.87 + 2.04 mu g/L. Compared with the 1,2,3 group, the mean value was higher than the average of the previous three groups. (2) the mean of the asthma group was 6.14 + 0.35 mu g/L, the mean of the eczema group was 2.92 + 1.17 g/L, and the mean of the nephritis group was + + g/L, and the average of the rheumatoid arthritis group was The mean value of.42 + 12.52 mu g/L, the mean of liver cirrhosis was 3.13 + 1.73g/L, the mean value of the normal people was 3.06 + 1.53 mu g/L. asthma and rheumatoid arthritis, the mean value was higher than that of the normal people. The mean value of the patients with eczema, nephritis and liver cirrhosis was not significantly different from those of the normal people. (3) the content of trypsin like trypsin in the heart and blood of 4 cadavers was 24 g/L, 6.7 respectively. 1 mu g/L, 4.83 mu g/L, 19.7 mu g/L.
Conclusions: (1) the content of trypsin like tryptase in the serum of Chinese immune normal population is 2.69 g/L, and the normal range is [0,6.00] g/L. without sex difference, but the age factors have some influence on it. (2) the serum level of leuzase in serum of patients with asthma and rheumatoid arthritis is higher than that of normal people, eczema, nephritis and cirrhosis of the liver. There was no significant difference in the content of tryptase in the serum of the patients. (3) the content of trypsin like tryptase in the corpse of the dead body of drug and trauma was much higher than that of the normal person, while the content of the corpse died of atherosclerosis had no significant change.

【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R363

【参考文献】