神经干细胞在大鼠脊髓内存活及迁移的实验研究

发布时间：2018-04-21 18:09

本文选题：神经干细胞 + 体内移植　；参考：《吉林大学》2007年硕士论文

【摘要】： 神经干细胞(Neural stem cells, NSCs)是广泛存在于中枢神经系统内未分化的细胞,它具有自我增殖及多向分化的潜能,被移植回中枢神经系统后,能进一步迁移、分化,与宿主神经元整合并发挥神经功能。NSCs体内移植为神经系统损伤修复和退行性病变的治疗带来新的希望,从而成为当今神经科学研究的热点之一。本实验采用无血清培养技术培养NSCs,并利用神经干细胞的标记物对其进行鉴定,应用神经元、神经胶质细胞、少突胶质细胞特异性标志物NSE、GFAP、O4对自然分化的NSCs进行免疫细胞化学染色,证明其多向分化潜能,同时用免疫组织化学染色技术观察了移植至大鼠脊髓内的NSCs存活及迁移情况。结果显示:应用DMEM-F12培养基,内添加B27、EGF及bFGF进行NSCs的培养,获得的细胞球呈Nestin及Musashi染色阳性。细胞在添加10%胎牛血清自然分化后,呈NSE、GFAP、O4染色阳性。NSCs在大鼠体内移植2周BrdU免疫组化染色结果显示:在移植的脑脊膜处可见阳性细胞,散在分布脊髓白质内,脊髓中央管周围及灰质内亦可见阳性细胞。提示:本实验成功获得了神经干细胞,获得的神经干细胞可以向神经元、神经胶质细胞、少突胶质细胞分化。将NSCs移植后入大鼠脊髓内,NSCs可以存活并向周围迁移。
[Abstract]:Neural stem cells (NSCs) are undifferentiated cells in the central nervous system (CNS), which have the potential of self-proliferation and multi-differentiation, and can migrate and differentiate further after being transplanted back to the CNS. Integration with host neurons and transplantation of NSCs in vivo bring new hope for the repair of nervous system injury and the treatment of degenerative diseases, and thus become one of the hotspots in neuroscience research. In this experiment, NSCs were cultured by serum-free culture, and the neural stem cell markers were used to identify NSCs. Neurons and glial cells were used to identify NSCs. Oligodendrocyte specific marker NSE-GFAPO4 was used for immunocytochemical staining of naturally differentiated NSCs to prove its multidirectional differentiation potential. The survival and migration of NSCs transplanted into the spinal cord of rats were observed by immunohistochemical staining. The results showed that NSCs was cultured on DMEM-F12 medium supplemented with B27 EGF and bFGF, and the cells were positive for Nestin and Musashi staining. After adding 10% fetal bovine serum to natural differentiation, the cells showed NSE-GFAPO4 positive staining. The results of BrdU immunohistochemical staining showed that positive cells could be seen in the transplanted meninges and scattered in the white matter of spinal cord. Positive cells were also seen around the central canal of the spinal cord and in the gray matter. The results showed that the neural stem cells were successfully obtained, and the neural stem cells could differentiate into neurons, glial cells and oligodendrocytes. NSCs transplanted into rat spinal cord can survive and migrate around.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R329

【参考文献】