适于新型液态栓塞材料的侧壁型动脉瘤动物模型的制备

发布时间：2018-04-22 16:31

本文选题：液态栓塞材料 + 动脉瘤模型　；参考：《吉林大学》2007年硕士论文

【摘要】： 人类颅内动脉瘤(Aneurysm,AN)破裂出血常引发病人出现严重的神经系统症状导致残废或死亡,因此常被喻为人脑内的“定时炸弹”。其主要治疗方法有:外科开颅AN夹闭术和血管内介入栓塞治疗。血管内介入治疗因其无需开刀,创伤小痛苦少,术后恢复快日益被脑血管病患者接受,随着CT血管造影(Computed tomographic angiography,CTA)和磁共振血管造影(Magnetic resonance angiography, MRA)、数字减影血管造影( Digital subtraction angiography,DSA)等影像学检查手段的发展以及栓塞方法的进步,使得血管内介入治疗迅速发展,很多新的栓塞材料不断出现。成纤维细胞-海藻酸盐-热敏脂质体是一种新型液态温控式、具有生物活性组织修复功能的栓塞材料,符合栓塞材料未来的发展方向。但此种栓塞材料的研究目前只进行了体外实验,临床实际应用的各项指标还需在动物模型上进一步研究,为了给这种栓塞材料的进一步研究提供良好的AN动物模型,本次实验利用显微外科静脉囊移植法制备家猪侧壁型AN模型。通过显微外科技术直接将静脉囊吻合于颈总动脉(Common carotid artery,CCA)侧壁后,于术后1周行DSA检查,检测AN模型的建立是否成功,建立成功率75%;于术后4周再行DSA检查,以明确该AN模型的瘤腔内附壁血栓形成情况,仍有67%的AN模型保持瘤体、瘤颈结构,但均有不同程度附壁血栓形成。通过DSA对AN模型的评估,得出结论:采用显微外科手术静脉囊移植法技术建立家猪CCA侧壁型AN模型的方法经济可行;侧壁型AN模型的成功率高、制备操作简单高效,实验动物存活率高;便于液态栓塞材料血管内介入栓塞操作及术后观察;虽然模型检测手段各具特点,DSA为最佳评估检测方法,同时可以为新型液态栓塞材料实验进一步研究提供技术基础;虽然此种侧壁宽颈AN模型自身不适宜血管内栓塞的操作,但是我们利用球囊封堵技术辅助栓塞可以弥补其缺点。
[Abstract]:Rupture and hemorrhage of human intracranial aneurysm Aneurysman often lead to severe neurological symptoms, which lead to disability or death, so it is often referred to as a "time bomb" in human brain. The main treatment methods are: surgical closure of an and endovascular embolization. Intravascular interventional therapy is increasingly accepted by patients with cerebrovascular disease because it does not require surgery and has less pain and pain. With the development of imaging methods such as computed tomographic angiography (CTA), magnetic resonance angiography (MRAA), Digital subtraction angiography (DSAs) and the improvement of embolization methods, endovascular interventional therapy has developed rapidly. Many new embolic materials are emerging. Fibroblast-alginate-thermosensitive liposome is a new type of liquid thermo-controlled embolic material with bioactive tissue repair function, which is in line with the future development of embolic materials. However, the study of this kind of embolization material has only been carried out in vitro, and the indexes of clinical application need to be further studied on animal models. In order to provide a good an animal model for the further study of this kind of embolization material, In this experiment, we used microsurgical venous sac transplantation to prepare an model of swine lateral wall. The venous sac was directly anastomosed to the lateral wall of Common carotid Arteria arteriosus by microsurgical technique. DSA examination was performed at 1 week after operation to detect the success of an model, and the success rate was 75%, and the DSA examination was performed 4 weeks after operation. In order to identify the intraluminal thrombus formation in the an model, 67% of the an models remained the tumor and neck structure, but all had different degree of mural thrombosis. Based on the evaluation of an model by DSA, it is concluded that it is economical and feasible to use microsurgical venous sac transplantation technique to establish CCA side-wall an model in domestic pigs, the success rate of side-wall an model is high, and the preparation of side-wall an model is simple and efficient. The survival rate of experimental animals is high; it is convenient for intravascular embolization of liquid embolization materials and postoperative observation; although model detection methods have their own characteristics, DSA is the best method for evaluation and detection. At the same time, it can provide the technical foundation for the further study of the new liquid embolic material. Although the side-wall wide-neck an model is not suitable for the endovascular embolization, we can make up for its shortcomings by using the balloon occlusion technique to assist the embolization.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R-332

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