胆汁对体外细粒棘球蚴原头节的作用观察

发布时间：2018-04-23 17:07

本文选题：细粒棘球蚴 + 原头节　；参考：《中国病原生物学杂志》2014年01期

【摘要】：目的探讨不同浓度的胆汁对细粒棘球蚴原头节的生长作用及形态学影响。方法将体外培养的细粒棘球蚴原头节分别加入40%、60%、80%、100%的胆汁中体外孵育。0.1%伊红染色在倒置显微镜下检测原头节的活力及形态改变,实验重复三次;透射电子显微镜下(TEM)下观察胆汁作用后原头节表面超微结构改变。结果不同浓度的胆汁对原头节均有杀伤作用,其中浓度为100%和80%的胆汁对细粒棘球蚴原头节杀伤作用最明显。倒置显微镜下观察发现,不同浓度的胆汁作用细粒棘球蚴原头节后,原头节多呈外翻型,原头节顶突上的小钩排列紊乱,部分脱落,吸盘突起,变形,活动性减弱。随着浓度的增加,变化越明显。超微结构显示40%的胆汁作用3d后,原头节纤毛出现少量缺损,合胞体带排列较紊乱,合胞体带内出现散在少量的空泡和脂滴。60%胆汁作用3d后,合胞体带内微毛出现融合,腔内出现空泡和脂滴,数量多且大。结论胆汁作用后,可导致体外细粒棘球蚴原头节的形态发生不同程度的改变并有有抑制作用,然而关于胆汁在体内的疗效有待于进一步研究。
[Abstract]:Objective to investigate the effect of bile at different concentrations on the growth and morphology of echinococcus granulosus. The ultrastructural changes of the surface of the primary ganglion were observed under TEM (Tem). Results Bile of different concentration had killing effect on the original ganglia, of which 100% and 80% bile had the most obvious killing effect on the procephalus of echinococcus granulosus. With the increase of concentration, the change is more obvious. Vacuoles and lipid droplets appeared in the cavity. Conclusion Bile treatment can result in different degrees of morphogenesis and inhibition of echinococcus granulosus in vitro. However, the effect of bile in vivo needs further study.
【作者单位】：石河子大学医学院组织胚胎学教研室;石河子大学第一附属医院肝胆外科;
【基金】：国家自然科学基金项目(No.30960338,81360410,U1303121) 兵团博士基金项目(No.2011BB019) 兵团重点领域科技攻关(No.2011BA058)
【分类号】：R383.33

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