脑活素、NGF对人类缺氧缺血SVZ的神经保护

发布时间：2018-04-24 14:13

本文选题：人类胚胎脑 + SVZ　；参考：《福建医科大学》2006年硕士论文

【摘要】： 目的研究缺氧缺血(hypoxic ischemic, HI)对妊娠中期人类胚胎脑脑室管膜下区(subventricular zone, SVZ)神经干细胞(neural stem cells,NSCs)、神经元、神经胶质细胞(星形胶质细胞、少突胶质祖细胞和小胶质细胞)的影响,比较不同细胞对HI损伤的耐受性差异,揭示缺氧缺血脑损伤(Hypoxic-ischemic brain damaged , HIBD)及其它发育脑损伤性疾病的多种脑功能障碍表现的细胞学基础。探讨脑活素、NGF对HI损伤的SVZ神经细胞的保护作用,为临床用药提供实验及理论依据。方法取妊娠17~22孕周(gestational weeks,gw)的健康胚胎脑5例,即刻解离出SVZ细胞,以氧糖缺失法(oxygen/glucose deprivation, OGD)[1]建立HI损伤模型,计算HI组和正常组细胞成活率。将HI组和正常组SVZ细胞按A药(NGF)、B药(脑活素)两因素,用药、不用药两个水平,交叉分成4组培养,培养4小时后以细胞特异性标志蛋白nestin、MAP2、GFAP、PDGFRα及RCA120的抗体通过免疫荧光细胞化学法鉴定SVZ的5种主要细胞:NSCs、神经元、星形胶质细胞、少突胶质祖细胞及小胶质细胞。比较HI组和正常组5种细胞含量的改变,分析两药对HI和正常组SVZ细胞总数、对HI组SVZ神经元数的影响。结果妊娠中期人类胚胎脑SVZ中含有15.08±0.02%的nestin(+)NSCs、48.81±0.03%的MAP2(+)神经元、32.31±0.03%的GFAP(+)星形胶质细胞、6.92±0.02%的PDGFRα(+)少突胶质祖细胞和1.15±0.01%的RCA120(+)小胶质细胞。细胞成活率:HI组为63.41±0.06%,正常组为98.9±0.01%。HI组细胞成活率明显低于正常组,差异具有显著性(p0.001),提示HI后SVZ细胞损伤明显。 5种SVZ细胞的百分含量结果比较:nestin(+)的NSCs分别占HI组和正常
[Abstract]:Objective to study the effects of hypoxic ischemia (HIH) on neural stem cells, neurons and glial cells (astrocytes, oligodendrocytes and microglia) in the subventricular zone of human embryonic brain in the second trimester of pregnancy. By comparing the tolerance of different cells to HI injury, the cytological basis of Hypoxic-ischemic brain damaged (HIBDs) and other developmental brain damage disorders was revealed. To investigate the protective effect of brain activin on SVZ neurons injured by HI, and to provide experimental and theoretical basis for clinical use of NGF. Methods SVZ cells were dissociated from 5 healthy fetal brains of 17 weeks gestational weeks gestational week gestational week gestational gestational week of pregnancy. Oxygen-glucose deprivation (OGD1) was used to establish HI injury model, and the survival rate of HI group and normal group were calculated. The SVZ cells of HI group and normal group were divided into 4 groups according to the two factors of A drug NGF FG B (cerebrolysin), two levels of no medication and four groups of culture. After 4 hours of culture, the five major cells of SVZ were identified by immunocytochemistry with the antibodies to the cell specific marker protein nestin-MAP2GFAPR 伪 and RCA120. The five main cells of SVZ were identified as: NSCs, neurons, astrocytes, oligodendrocyte progenitors and microglial cells. The changes of 5 kinds of cells in HI group and normal group were compared, and the effects of the two drugs on the total number of SVZ cells and the number of SVZ neurons in HI group were analyzed. Results there were 15.08 卤0.02% nestin-like neurons and 32.31 卤0.03% astrocytes and 6.92 卤0.02% PDGFR 伪 (oligodendrocyte) and 1.15 卤0.01% RCA120 (microglia) in human embryonic brain SVZ in the second trimester of pregnancy. The survival rate of SVZ cells in HI group (63.41 卤0.06) and normal group (98.9 卤0.01%.HI) was significantly lower than that in normal group (P 0.001). Comparison of the percentage content of five kinds of SVZ cells in HI group and normal group
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R714.7;R363

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