元素镧和氟对动物免疫和内分泌的影响

发布时间：2018-04-24 21:21

本文选题：镧 + 氟　；参考：《安徽医科大学》2007年硕士论文

【摘要】： 1稀土元素镧对大鼠免疫功能的影响目的探讨硝酸镧对大鼠的淋巴细胞转化率(LTR)、红细胞免疫(红细胞C_(3b)受体花环率,RC_(3b)RR)和巨噬细胞活性(PPM)等免疫功能的影响。方法64只40日龄的SD雄性大鼠,随机分为实验组Ⅰ,实验组Ⅱ,实验组Ⅲ和对照组,每组16只。实验组灌饲硝酸镧溶液,剂量依次为0.1,2,20mg/kg体重,每只2ml,1次/天。对照组灌饲生理盐水,2ml/只,1次/天。10天后各组处死一半(共32只)做短期研究,30天后处死剩下的一半做长期研究。比较在十天(短期影响)和三十天(长期影响)后,各组大鼠的淋巴细胞转化率、红细胞C_(3b)受体花环率和巨噬细胞活性,并进行差异的显著性检验。结果短期影响:中等剂量的硝酸镧(2mg/kg)组(实验Ⅱ组)腹腔巨噬细胞吞噬活性有显著提高(P＜0.05),而淋巴细胞转化率,红细胞C_(3b)受体花环率有提高趋势,但未达到显著水平(P＞0.05)。长期影响:低剂量(0.1mg/kg)的硝酸镧组(实验Ⅰ组)红细胞C_(3b)受体花环率高于对照组(P＜0.05),而淋巴细胞转化率和腹腔巨噬细胞吞噬活性均呈上升趋势,但未达到显著水平(P＞0.05);中等剂量(2mg/kg)的硝酸镧组(实验Ⅱ组)淋巴细胞转化率、红细胞C_(3b)受体花环率及其腹腔巨噬细胞吞噬活性均显著高于对照组(P＜0.05);高等剂量(20mg/kg body weight)的硝酸镧组(实验Ⅲ组)淋巴细胞转化率和腹腔巨噬细胞吞噬活性均低于对照组,但未达到显著水平(P＞0.05)。结论大鼠饲喂硝酸镧溶液在一定的时间和一定的剂量范围内能够提高其免疫能力;中、低剂量的硝酸镧对大鼠的免疫力有促进作用,高剂量时则有抑制趋势。 2稀土元素镧对大鼠内分泌功能的影响目的探讨硝酸镧对大鼠内分泌功能的影响。方法64只40日龄的SD雄性大鼠,随机分为实验组Ⅰ,实验组Ⅱ,实验组Ⅲ和对照组,每组16只。实验组灌饲硝酸镧溶液,剂量依次为0.1,2,20mg/kg体重,每只2ml,1次/天。对照组灌饲生理盐水,2ml/只,1次/天。10天后各组处死一半(共32只)做短期研究,30天后处死剩下的一半做长期研究。比较在十天(短期影响)和三十天(长期影响)后,各组大鼠血清中甲状腺激素(T_3、T_4)和胰岛素(Ins)水平,并进行差异的显著性检验。结果短期影响:大鼠灌喂硝酸镧,胰岛素水平变化明显,低剂量组(0.1mg/kg)、中剂量组(2mg/kg)和高剂量组(20mg/kg)的胰岛素水平比对照组均有显著升高(P＜0.05);甲状腺激素(T_3、T_4)变化表现出低剂量组(0.1mg/kg)T_3分泌水平提高(P＜0.05)。长期影响:大鼠灌喂硝酸镧,胰岛素水平变化也非常明显,低剂量组(0.1mg/kg)、中剂量组(2mg/kg)和高剂量组(20mg/kg)的胰岛素水平比对照组均有显著升高(P＜0.05):甲状腺激素(T_3、T_4)无明显变化(P＞0.05)。结论硝酸镧对大鼠甲状腺激素(T_3、T_4)的分泌无明显影响,而显著提高大鼠胰岛素的分泌。 3微量元素氟对小鼠内分泌功能的影响目的探讨微量元素氟及高氟补硒后对小鼠甲状腺、胰岛等内分泌功能的影响。方法将80只昆明种小鼠(19-22g)随机分为随机分为8组,短期和长期各四组,这四组分别为对照组、实验Ⅰ组(低氟组)、实验Ⅱ组(高氟组)、实验Ⅲ组(高氟+硒组)。10只/组。三个实验组小鼠分别腹腔注射20mg/L氟化纳溶液、200mg/L氟化纳溶液以及200mg/L氟化纳与100mg/L亚硒酸纳的混合溶液,对照组小鼠腹腔注射生理盐水。每只小鼠每天注射一次,每次1ml,短期实验10天,长期实验30天。比较在十天(短期影响)和三十天(长期影响)后,比较组小鼠血清中T_3、T_4及Ins水平的变化,并进行差异的显著性检验。结果短期影响:低氟组T_3、T_4及Ins水平与对照组相比,无显著差异(P＞0.05);高氟组T_4和Ins水平高于对照组,有显著差异(P＜0.05),T_3水平有升高趋势,但无显著差异(P＞0.05);高氟加硒组T_3、T_4及Ins水平与高氟组比较均有下降趋势。长期影响:低氟组T_3、T_4及Ins水平与对照组相比,无显著差异(P＞0.05);高氟组T_4和Ins水平低于对照组,有显著差异(P＜0.05),T_3水平有下降趋势,但无显著差异(P＞0.05);高氟加硒组T_3、T_4水平与高氟组比较均有上升趋势,Ins水平与高氟组比较有下降趋势。结论高氟状态可影响机体甲状腺、胰岛等内分泌腺体的分泌功能。短期使甲状腺兴奋,分泌功能增强,长期则抑制甲状腺功能使其分泌功能减弱;高氟对胰岛的分泌功能有刺激作用,可使其功能增强、胰岛素分泌增加;甲状腺、胰岛等内分泌腺功能的改变与氟的摄入量及摄入时间的长短存在一定关系;在高氟状态下补硒后,使甲状腺、胰岛等内分泌腺功能改变呈回复趋势。
[Abstract]:The effect of Lanthanum on immune function of rats with 1 lanthanum
Objective to investigate the effects of lanthanum nitrate on lymphocyte transformation rate (LTR), red cell C_ (3b) receptor rosette rate, RC_ (3b) RR) and macrophage activity (PPM). Methods 64 40 day old SD male rats were randomly divided into experimental group I, experimental group II, experimental group III and control group, 16 rats in each group. The experimental group was fed with nitrate. The dose of lanthanum acid was 0.1,2,20mg/kg weight, each 2ml, 1 times per day. The control group was fed with physiological saline, 2ml/ only, 1 times / day.10 days were killed in half (a total of 32) for short-term study. 30 days later, the remaining half of the study was done. After ten days (short-term effect) and thirty days (long-term effect), the lymphocyte turns in each group. Rate, red cell C_ (3b) receptor rosette rate and macrophage activity, and the difference between the significant test. Results short term effect: the phagocytic activity of peritoneal macrophages in the medium dose (2mg/kg) group (experimental group II) increased significantly (P < 0.05), and the lymphatic cell transformation rate, the red cell C_ (3b) receptor rosette rate increased, but not reached. To a significant level (P > 0.05). Long term effect: the rate of red blood cell C_ (3b) receptor rosette in the low dose (0.1mg/kg) group of lanthanum nitrate (experimental group I) was higher than that of the control group (P < 0.05), but the lymphocyte transformation rate and the phagocytic activity of peritoneal macrophages were all rising, but not reached the significant level (P > 0.05), and the medium dose (2mg/kg) lanthanum nitrate group (Experiment II) Lymphocyte transformation rate, erythrocyte C_ (3b) receptor rosette rate and phagocytic activity of peritoneal macrophage were significantly higher than that of control group (P < 0.05). The lymphocyte transformation rate and phagocytic activity of peritoneal macrophages in the high dose (20mg/kg body weight) group of lanthanum nitrate group (experimental group III) were lower than those of the control group, but did not reach the significant level (P > 0.05). Conclusion the rats fed with lanthanum nitrate can improve their immune ability in a certain time and a certain dose range, and the low dose of lanthanum nitrate can promote the immunity of rats, and there is a trend of inhibition in high dose.
The effect of Lanthanum on the endocrine function of rats with 2 lanthanum
Objective to investigate the effect of lanthanum nitrate on the endocrine function of rats. Methods 64 SD male rats aged 40 days old were randomly divided into experimental group I, experimental group II, experimental group III and control group, 16 rats in each group. The experimental group was fed with lanthanum nitrate solution in order of 0.1,2,20mg/kg weight, each 2ml, 1 times. The control group was fed with physiological saline, 2ml/ only, 1 times / day.10 Half of each group died (32) for short term study. 30 days later, half of the rest were executed for long-term study. After ten days (short-term impact) and thirty days (long term effect), the serum levels of thyroid hormone (T_3, T_4) and insulin (Ins) were compared and the difference was observed. Results the short-term effect: rats fed with lanthanum nitrate, The insulin level in the low dose group (0.1mg/kg), the middle dose group (2mg/kg) and the high dose group (20mg/kg) were significantly higher than the control group (P < 0.05). The changes of thyroid hormone (T_3, T_4) showed the level of T_3 secreting in the low dose group (P < 0.05) (P < 0.05). The long-term effect: rats fed with lanthanum nitrate and insulin level The changes in the low dose group (0.1mg/kg), the middle dose group (2mg/kg) and the high dose group (20mg/kg) were significantly higher than those in the control group (P < 0.05): thyroid hormone (T_3, T_4) had no significant changes (P > 0.05). Conclusion lanthanum nitrate had no significant effect on the secretion of thyroid hormone (T_3, T_4) in rats, and significantly increased the islet of rats. The secretion of a hormone.
The effect of 3 trace element fluorine on the endocrine function of mice
Objective to investigate the effect of trace element fluorine and high fluorine supplementation on the endocrine function of the thyroid and islets of mice. Methods 80 Kunming mice (19-22g) were randomly divided into 8 groups, the short-term and the long-term four groups, the four groups were the control group, experimental group I (low fluorine group), experimental group II (Gao Fuzu), experimental group III (high fluoride + Se group).10 only / The mice in the three experimental groups were injected intraperitoneally with 20mg/L fluorine sodium solution, 200mg/L fluorine sodium solution and 200mg/L fluorine sodium and 100mg/L selenite. The control mice were injected intraperitoneally with saline. Each mouse was injected once a day, each time was 1ml, the short-term experiment was 10 days, and the long-term experiment was 30 days. The comparison was compared to ten days (short-term effect) and three. After ten days (long term influence), the changes of T_3, T_4 and Ins levels in the serum of mice were compared, and the significant difference between the levels of T_3, T_4 and Ins in the low fluorine group was not significant (P > 0.05), and the level of T_4 and Ins in the high fluorine group was higher than that in the control group (P < 0.05), but the T_3 level had a rising trend, but the level of T_3 was higher than that of the control group, but the level of T_3 was higher than that in the control group. There was no significant difference (P > 0.05). The level of T_3, T_4 and Ins in the high fluoride and selenium group had a downward trend compared with the high fluorine group. The long-term effect: the level of T_3, T_4 and Ins in the low fluorine group was no significant difference compared with the control group (P > 0.05), and the level of T_4 and Ins in the high fluorine group was lower than that of the control group (P < 0.05), and the T_3 level declined, but there was no significant difference (0. > > > > > > > > > > > > 0.. 05): the level of T_3 and T_4 in the high fluoride and selenium group had a rising trend compared with the high fluorine group. The level of Ins and the high fluorine group had a downward trend. Conclusion the high fluorine state could affect the secretory function of the thyroid gland and the pancreatic islets. The thyroid gland was excited and the secretory function was enhanced in the short term, and the secretion function of the thyroid gland was weakened for a long time. Fluorine has a stimulating effect on the secretory function of islets, which can enhance its function, increase the secretion of insulin, change the function of endocrine glands such as thyroid and islet, and have a certain relationship with the intake of fluorine and the length of the intake time.

【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R363

【参考文献】