NF-κB信号通路对BLP耐受巨噬细胞iNOS表达的影响

发布时间：2018-04-25 09:15

本文选题：BLP耐受 + 细菌吞噬　；参考：《中国临床解剖学杂志》2014年03期

【摘要】：目的通过检测细菌脂蛋白(BLP)耐受巨噬细胞感染细菌时诱导型一氧化氮合酶(iNOS)表达情况及其表达是否受NF-κB信号通路调控,探讨BLP耐受巨噬细胞对细菌清除能力增强的机制。方法比较BLP耐受和非耐受(Naive)小鼠骨髓来源的巨噬细胞(BMM)对大肠杆菌的吞噬及杀灭情况,评价BLP耐受巨噬细胞的细菌清除能力;用定量PCR技术检测BLP耐受的BMM内iNOS mRNA表达情况和细胞免疫荧光技术观察p65从胞质向胞核的移位情况;最后观察抑制NF-κB通路活化对iNOS mRNA表达的影响。结果 BLP耐受巨噬细胞吞噬细菌和杀灭细菌的能力较Naive细胞显著增强(P0.05);iNOS mRNA表达水平较Naive细胞显著(P0.05);如果抑制BLP耐受巨噬细胞NF-κB通路活化对iNOS mRNA表达有显著影响(P0.05)。结论本研究结果提示细菌脂蛋白耐受通过NF-κB通路活化增强细菌感染巨噬细胞iNOS表达。
[Abstract]:Objective to investigate the mechanism of BLP tolerant macrophages by detecting the expression of inducible nitric oxide synthase (iNOS) and whether its expression is regulated by NF- 魏 B signaling pathway. Methods to compare the phagocytosis and killing effect of macrophages derived from bone marrow of BLP tolerant and intolerant mice on Escherichia coli, and to evaluate the bacterial clearance ability of BLP tolerant macrophages. The expression of iNOS mRNA in BLP tolerant BMM and the translocation of p65 from cytoplasm to nucleus were detected by quantitative PCR technique, and the effect of inhibiting the activation of NF- 魏 B pathway on the expression of iNOS mRNA was observed. Results the ability of BLP to tolerate macrophages phagocytosis and kill bacteria was significantly enhanced than that of Naive cells, and the expression of P0.05 mRNA in BLP tolerant macrophages was significantly higher than that in Naive cells, and if the activation of NF- 魏 B pathway in BLP tolerant macrophages was inhibited, the expression of iNOS mRNA was significantly affected. Conclusion this study suggests that bacterial lipoprotein tolerance enhances the expression of iNOS in macrophages infected by bacteria through NF- 魏 B pathway activation.
【作者单位】：广东省蛋白质组学重点实验室南方医科大学病理生理学教研室;
【基金】：国家自然科学基金(81272149,81072425)
【分类号】：R363

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