小鼠颌骨发育过程中长链非编码RNA的表达谱变化

发布时间：2018-04-27 20:23

本文选题：颌骨发育 + 长链非编码RNA　；参考：《中华口腔医学研究杂志(电子版)》2014年06期

【摘要】：目的研究在小鼠颌骨发育过程中长链非编码RNA(lnc RNA)的表达谱变化情况。方法利用lnc RNA-seq测序技术检测孕18 d及出生后14 d的C57小鼠下颌骨组织样本中的lnc RNA表达谱差异,经对原始数据进行预处理达到均一化后,筛选出差异表达lnc RNA,进行分析。结果孕18 d与出生后14 d的C57小鼠下颌骨组织样本相比较,2倍以上变化并有显著差异(FDR≤0.001)的lnc RNA,确定为差异表达lnc RNA。2倍以上变化的共6617条,占所有lnc RNA的17.16%;2倍以上升高的共3720条;2倍以上降低的共2897条;5倍以上升高的共714条;5倍以上降低的共288条;10倍以上升高为共645条;10倍以上降低的共211条。结论孕18 d与出生后14 d的C57小鼠下颌骨组织样本相比较,lnc RNA表达谱发生显著变化。提示差异性表达的lnc RNA可能参与了小鼠颌骨发育的调控。
[Abstract]:Objective to study the expression profile of long chain non coding RNA(lnc during the development of mouse jaw. Methods lnc RNA-seq sequencing technique was used to detect the difference of lnc RNA expression profile in the mandibular tissue samples of C57 mice on the 18th day of pregnancy and 14 days after birth. After homogenization of the original data, differential expression lnc was screened and analyzed. Results compared with those of C57 mice on the 14th day of gestation, there were more than 2 times changes in the mandibular tissue samples and there were significant differences in lnc RNAs between them. A total of 6617 lnc RNAs were identified as differential expression of lnc RNA.2. The total number of 3720 more than twice increased in all lnc RNA was more than 2 times that of 2897 more than 5 times higher than that of 2897 and more than 5 times higher. The total number of more than 10 times lower than 10 times of the total number of lnc RNA was increased to that of the total of 645 or more than 10 times of the decrease of the total number of 211. Conclusion the expression profile of LNC RNA in mandibular tissues of C57 mice on the 18th day of gestation was significantly different from that of the C57 mice on the 14th day of gestation. These results suggest that the differentially expressed lnc RNA may be involved in the regulation of jaw development in mice.
【作者单位】：中山大学孙逸仙纪念医院口腔科;
【基金】：国家自然科学基金青年科学基金项目(81200825)
【分类号】：R333.1

【共引文献】