Rh血型基因定型方法的实验研究

发布时间：2018-04-28 23:04

本文选题：Rh血型 + 序列特异性聚合酶链式反应(PCR-SSP)　；参考：《吉林大学》2007年硕士论文

【摘要】： Rh血型系统是临床输血医学中仅次于ABO血型系统的输血因子,在人类血型系统中最为复杂的当属Rh血型系统,它能导致溶血性输血反应和新生儿溶血病[1]。在临床输血工作中,对Rh血型的分型使用血清学方法,根据红细胞上D抗原的有无,分为Rh阴性和Rh阳性。据报导Rh阳性在我国汉族及大多数民族中约占99.6%,个别少数民族为90%。近年来,随着分子生物学研究的不断深入,对Rh血型基因的研究广泛开展,其中研究最多的是序列特异性聚合酶链式反应(PCR-SSP),即根据RH基因的特异性序列,通过PCR扩增出特异性产物,研究Rh血型基因的多态性。本实验采用PCR-SSP法,通过对Rh血型基因特异性序列的扩增,分析RH基因多态性,旨在建立适合Rh血型的基因定型方法,为应用于临床奠定基础。
[Abstract]:Rh blood group system is the second only blood transfusion factor in clinical transfusion medicine after ABO blood group system. The most complex blood group system in human blood group system is Rh blood group system, which can cause hemolytic transfusion reaction and neonatal hemolytic disease [1]. In clinical blood transfusion, serological method was used to classify Rh blood group into Rh negative and Rh positive according to the presence or absence of D antigen on erythrocytes. Rh positive is reported to be about 99.6% of the Han and most ethnic groups in China, and 90% for individual minorities. In recent years, with the development of molecular biology, the research of Rh blood group gene has been carried out extensively, among which the sequence specific polymerase chain reaction (PCR-SSPN) is the most widely studied, that is, according to the specific sequence of RH gene, The specific products were amplified by PCR to study the polymorphism of Rh blood group gene. In this study, PCR-SSP method was used to amplify the specific sequence of Rh blood group gene and to analyze the polymorphism of RH gene. The aim of this study was to establish a gene typing method suitable for Rh blood group and to lay a foundation for clinical application.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R392

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