长角血蜱雌蜱唾液腺蛋白质及激素调控的研究

发布时间：2018-04-29 00:37

  本文选题：长角血蜱 + 雌蜱　； 参考：《河北师范大学》2005年博士论文

【摘要】：蜱类是专性吸血的外寄生节肢动物,传播多种人、畜疾病,给人类健康和畜牧业发展带来极大危害。唾液腺是蜱体内的一个重要器官,在其生命活动中起重要作用。它不仅能维持体内离子、水分平衡,使蜱成功吸血,而且又是各类疾病传播的窗口。因此,对其研究具有重要的理论和实践意义。 本文以广泛分布于我国的长角血蜱Haemaphysalis longicornis Neumann雌蜱为研究对象,利用生物化学、激素研究和蛋白质组学等现代生物学前沿技术对不同发育期唾液腺蛋白质的动态变化、保幼激素和蜕皮激素对唾液腺是否有影响等方面进行了较为系统的研究,并对雌蜱唾液腺蛋白质组进行了初步分析,填补了该领域中的不足,为深入开展蜱类唾液腺研究提供了依据。主要研究结果如下: 1.唾液腺蛋白含量在不同发育期发生变化。饥饿期蛋白含量低(9.51 μg/tick);吸血2d蛋白含量急剧增加(34.90 μg/tick);交配期达到峰值(84.40 μg/tick),约为饥饿期的8.8倍;饱血后蛋白含量明显下降,饱血后2d和饱血后4d的含量分别为39.47 μg/tick和20.54 μg/tick。 饥饿雌蜱唾液腺有17条蛋白带;吸血2d增至23条;交配后达到26条,直至饱血后4d保持不变,吸血和交配均能刺激新蛋白的合成。各期唾液腺蛋白中有主带10条,分子量分别为192 kDa、158 kDa、136 kDa、133 kDa、129 kDa、97 kDa、84 kDa、74 kDa、45 kDa和32 kDa,主带在不同发育期发生增加、缺失和量的变化。 2.保幼激素(JH Ⅲ)对雌蜱唾液腺蛋白含量和成分有作用,并随发育期和剂量的不同而产生不同效应。吸血2d用JH Ⅲ处理,10μg剂量使交配期唾液腺蛋白含量明显增加,10μg、50μg和100μg剂量抑制112 kDa蛋白基因的表达;饱血当天处理,10μg、50μg和100μg剂量均能显著提高饱血后2d唾液腺蛋白含量,100μg剂量使28 kDa蛋白基因表达和67 kDa蛋白基因上调表达,高剂量200μg和400μg使236 kDa和112 kDa的蛋白缺失;饱血当天处理,1μg和10μg剂量均能显著提高饱血后4d雌蜱唾液腺蛋白含量,400μg剂量则显著降低饱血后4d雌蜱唾液腺蛋白含量,100μg、200μg和400μg
[Abstract]:Tick is a special blood sucking parasite arthropod, spreading a variety of human and animal diseases, to human health and the development of animal husbandry bring great harm. Salivary gland is an important organ in ticks and plays an important role in their life activities. It can not only maintain the ion and water balance in the body and make ticks absorb blood successfully, but also serve as a window for the spread of various diseases. Therefore, its research has important theoretical and practical significance. The dynamic changes of salivary gland proteins in different developmental stages of Haemaphysalis longicornis (Haemaphysalis longicornis Neumann) were studied by using modern biological frontier techniques, such as biochemistry, hormone research and proteomics, which were widely distributed in China. The effects of juvenile hormone and ecdysone on salivary gland were studied systematically, and the proteome of female salivary gland was analyzed, which filled the deficiency in this field. It provides a basis for the further study of tick salivary glands. The main findings are as follows: 1. The content of salivary gland protein changes at different stages of development. The protein content in hunger stage was 9.51 渭 g / tickg, the protein content in 2-day blood sucking was increased sharply (34.90 渭 g / kg), the peak value of protein in mating stage was 84.40 渭 g / kg / kg, which was 8.8 times higher than that in starvation period, the content of protein decreased obviously after feeding, the content of protein was 39.47 渭 g/tick and 20.54 渭 g / kg / kg after feeding 2 days and 4 days after feeding, respectively. There were 17 protein bands in the salivary glands of female starved ticks, 23 in 2 days after feeding, 26 after mating, and no change until 4 days after feeding. Both bloodsucking and mating could stimulate the synthesis of new proteins. There were 10 main bands in the salivary gland proteins in each stage, the molecular weight of which was 192kDa 158kDa 158kDa 136kDa 133kDa-129kDa-129kDa-97 kDa-84kDa-74 kDa-45 kDa and 32kDa. the main bands increased, deletion and quantity changed at different stages of development. 2. The effect of JH 鈪，

本文编号：1817621