急性亚段肺动脉栓塞动物模型制备和影像诊断比较研究

发布时间：2018-04-29 01:06

本文选题：猪 + 急性亚段肺动脉栓塞　；参考：《南京医科大学》2006年硕士论文

【摘要】：目的：1．运用介入栓塞技术建立一种能用于影像学诊断的猪急性亚段肺动脉栓塞模型，并评价其技术上的可行性和准确性；2．比较多层螺旋CT肺动脉造影不同层厚对亚段肺动脉栓塞的显示能力；3．以肺动脉血管铸型标本为金标准，比较多层螺旋CT肺动脉造影、数字减影X线肺动脉造影对亚段肺动脉栓塞的显示能力。方法：1．5只家猪经右侧颈外静脉注入若干聚氯乙烯(PVC)栓塞剂(直径2-3mm)，栓塞前后分别做DSPA和CTPA。栓塞成功后24小时内处死动物，取出完整猪肺组织。经肺动脉主干注入适量铸型剂(32.8％树脂溶液)行血管铸型；2．5只家猪，均用1.0mm准直扫描进行CT肺动脉造影，分别用1.0mm／0.5mm(层厚／层间距)(A组)，2.0mm／1.0mm(B组)，，3.0mm／1.5mm(C组)重建，记录每只家猪3种不同重建层厚显示亚段肺动脉栓塞的敏感性和阳性预测值；3．5只家猪，分别进行数字减影X线肺动脉造影、多层螺旋CT肺动脉造影和肺动脉血管铸型，多层螺旋CT肺动脉造影图像分别用1mm、2mm、3mm层厚重建，记录每只家猪3种不同方法检测亚段肺动脉栓子的敏感性和阳性预测值。结果：1．5只猪全部成功制作急性亚段肺动脉栓塞模型，在DSPA图像上，亚段肺动脉栓塞表现为相应区域的肺动脉分支数减少；在CTPA
[Abstract]:Objective: 1. to establish a model of acute subsegmental pulmonary embolism that can be used for imaging diagnosis by interventional embolization, and to evaluate its technical feasibility and accuracy; 2. compare the ability to display subsegmental pulmonary embolism with different thickness of multi slice spiral CT pulmonary arteriography, and 3. with pulmonary artery vascular cast specimen as gold standard. Comparison of multislice spiral CT pulmonary angiography and digital subtraction pulmonary angiography in demonstrating the ability of subsegmental pulmonary embolism.
Methods: 1.5 domestic pigs were injected with a number of polyvinyl chloride (PVC) embolic agents (diameter 2-3mm) through the right external jugular vein. The animals were killed within 24 hours after embolization with DSPA and CTPA. respectively. The whole lung tissue was taken out. A proper amount of mould agent (32.8% tree fat solution) was injected into the main trunk of the pulmonary artery, and 2.5 domestic pigs were treated with 1.0mm. CT pulmonary angiography was performed by collimated scanning, and the sensitivity and positive predictive value of 3 different reconstructive layer thickness of each pig were recorded with 1.0mm / 0.5mm (layer thickness / interval) (group A), 2.0mm / 1.0mm (group B), 3.0mm / 1.5mm (C group), and 3.5 domestic pigs, respectively, with digital subtraction X-ray pulmonary angiography and multi layer snail, respectively. Spiral CT pulmonary angiography and pulmonary artery cast, multi-slice spiral CT pulmonary angiography images were reconstructed with 1mm, 2mm, 3mm thickness respectively. The sensitivity and positive predictive value of 3 different methods for detecting subsegmental pulmonary emboli were recorded in each pig.
Results: all 1.5 pigs successfully made an acute subsegmental pulmonary embolism model. In the DSPA image, the number of pulmonary artery branches in the corresponding region was reduced by subsegmental pulmonary artery embolization; in CTPA

【学位授予单位】：南京医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R-332;R816.4

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