SARS冠状病毒S2基因的克隆表达和S2蛋白抗原活性的初步研究

发布时间：2018-04-29 10:03

本文选题：SARS冠状病毒 + S2蛋白　；参考：《第一军医大学》2006年硕士论文

【摘要】：一、研究背景和目的严重急性呼吸综合征(sever acute respiratory syndrome，SARS)又称传染性非典型肺炎，是2003年新发现的一种呼吸系统传染病，其病原体是SARS冠状病毒(SARS coronavirus，SARS-CoV)。 SARS-CoV属于巢状病毒目(Nidovirales)、冠状病毒科(coronavir)，为单正链RNA病毒，基因组全长约29.7kb，，已知编码11种蛋白，其中包括4种结构蛋白，分别是刺突蛋白(S)、包膜蛋白(M)、小包膜蛋白(E)和核蛋白或核壳体蛋白(N)。S蛋白三聚体形成突起，伸出包膜表面。S蛋白是病毒的主要表面抗原成分，具有受体结合和膜融合活性，在组织嗜性、细胞融合和毒力等方面起重要作用。N蛋白与基因组RNA结合形成核衣壳。M蛋白和E蛋白是包膜形成所必需的成分。 S蛋白是I型膜糖蛋白，介导病毒与宿主细胞膜上的受体结合并与宿主细胞膜相融合。S蛋白由S1和S2两个亚基组成。S1形成球状部分，S2形成棒状部分，两者之间通过分子间的作用力相互结合。S1能与宿主细胞上的受体结合，S2可把整个S蛋白固定在细胞膜上，并介导病毒包膜与宿主细胞膜融合。S蛋白上有重要的病毒抗原决定簇，S基因的突变会影响病毒的致病性。 S蛋白由1256个氨基酸组成，有23个潜在的糖基化位点，其N端和C端相对保守。N端有由疏水氨基酸组成信号肽，剪切点可能位于第13或14位氨基酸，可协助病毒固定于内质网上。C端由高保守的跨膜区域和富含半胱氨酸
[Abstract]:I. background and purpose of the study Severe Acute Respiratory Syndrome (SARS), also known as infectious atypical pneumonia (SARS), is a newly discovered respiratory infectious disease in 2003. The pathogen of SARS coronavirus is SARS coronavirus (SARS-CoV). SARS-CoV belongs to Nidoviralesus, Coronavirus. It is a single positive strand RNA virus. Its genome length is about 29.7 kb. It is known to encode 11 proteins, including 4 structural proteins. The nucleocapsid or nuclear shell protein Nu. S protein trimer formed protuberances, respectively. The protruding surface protein of the envelope was the main surface antigen component of the virus, and had receptor binding and membrane fusion activity, and the nucleoprotein or nuclear shell protein (NU. S) was the main surface antigen component of the virus, and the nucleocapsid protein or nuclear shell protein (NU. S) was the main surface antigen of the virus. The nucleocapsid. M and E proteins play an important role in tissue eosinophilia, cell fusion and virulence. The nucleocapsid. M and E proteins are essential components for the formation of capsule. S protein is a type I membrane glycoprotein, which mediates the virus binding to the receptor on the host cell membrane and fuses with the host cell membrane. S protein consists of S1 and S2 subunits. S1 forms a spherical part and S 2 forms a rod part. Through the interaction between the two molecules. S1 can bind to the receptor on the host cell and S 2 can immobilize the whole S protein on the cell membrane. The mutation of virus antigen determinant S gene may affect the pathogenicity of virus by mediating the fusion of virus envelope and host cell membrane. S protein consists of 1256 amino acids with 23 potential glycosylation sites. The N-terminal and C-terminal have a signal peptide composed of hydrophobic amino acids, and the shear point may be located at the 13th or 14th amino acids. Can assist the virus to fix to the endoplasmic reticulum. C terminal by a highly conserved transmembrane region and rich in cysteine
【学位授予单位】：第一军医大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R373

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