高通量16S rRNA标签测序法比较人与不同动物肠道微生物组多样性

发布时间：2018-04-29 14:06

本文选题：微生物组 + Illumina测序　；参考：《生态科学》2014年05期

【摘要】：收集人、大猪、小猪、大鼠、小鼠以及鸡五个不同个体的粪便样品,每个个体取两个平行样,提取总DNA;PCR扩增,获得16S r RNA V6标签片段;Illumina测序;经BIPES以及QIIME分析并比较菌群结构及多样性。研究结果发现,不同物种之间肠道菌群差异较大。五类物种肠道菌群均以厚壁菌门、拟杆菌门、以及变形菌门为主,但鸡的厚壁菌门显著减少,而变形菌门显著增加。从?多样性角度来看,猪肠道菌群种属丰富度及Shannon指数均显著高于人及鸡肠道菌群。从?多样性角度,尽管不同人之间的肠道菌群相似度较低,但不同物种之间相比较,小猪与大鼠肠道菌群与人相似性高于小鼠和鸡肠道菌群。与人类相比,小猪的肠道微生物组最相近,而鸡的肠道菌群相似度最低。
[Abstract]:The fecal samples of human, pig, piglet, rat, mouse and chicken were collected. Two parallel samples were taken from each individual, and the total DNA-DNA fragment was amplified by PCR, and the 16s r RNA V6 tag fragment was sequenced. The flora structure and diversity were analyzed by BIPES and QIIME. The results showed that there were significant differences in intestinal flora among different species. The intestinal flora of five species were mainly phylum, Bacteroides and Proteus, but the phylum of Chicken decreased significantly, while the phylum of Proteus increased significantly. From? From the point of view of diversity, the abundance and Shannon index of pig intestinal flora were significantly higher than those of human and chicken intestinal flora. From? In terms of diversity, although the similarity of intestinal flora among different people was lower, the similarity between pig and rat intestinal flora was higher than that between mice and chicken. Compared with humans, pigs had the most similar intestinal microflora, while chickens had the lowest intestinal microflora similarity.
【作者单位】：广州市职业病防治院职业卫生评价检测中心广州市医学重点学科职业健康监护科广州市职业环境与健康效应实验室;南方医科大学公共卫生与热带医学学院;内蒙古自治区赤峰市疾病预防控制中心;
【基金】：国家自然科学基金面上项目(31270152) 广东省自然基金面上项目(S2011010004136) 广东省教育厅科研处科技创新项目(2012KJCX0031) 广州市“121人才梯队工程”后备人才项目
【分类号】：R37

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