甲型H1N1流感病毒基因特征及耐药性研究

发布时间：2018-04-29 13:42

本文选题：甲型HN流感病毒 + 基因序列分析　；参考：《中国病原生物学杂志》2014年09期

【摘要】：目的研究甲型H1N1流感病毒的基因特征和变化规律,为流感疫苗评价提供依据。方法选取2009年6月-2013年4月本院分离出的15株甲型H1N1病毒分离株,提取病毒RNA进行反转录。采用PCR方法扩增15株甲型H1N1流感病毒HA和NA全基因并进行测序分析。利用软件Bioedit和MEGA软件对序列进行拼接,绘制种系发生树,采用邻位相临法构建进化树。结果 15株甲型H1N1流感病毒均为低致病性病毒,对神经氨酸酶抑制剂敏感,对金刚烷胺类呈耐药性。实验毒株HA基因序列与国内和国外毒株同源性为99.1%~99.7%。该地区流感毒株抗原变异程度较大,其中毒株HA发生14个氨基酸点位改变,分别为:A215E,D127E,E235K,E374K,G170E,H138R,L161I,I321V,L191I,P83S,R45K,S185T,S203T和V234I。其中第83位和第321位与国内代表株相同,但是与国外代表株不同。NA发生15个氨基酸点位改变,分别为A20V,N44S,V83M,V106I,E128G,H144Y,I188T,V241I,S247N,N248D,S334N,N369K,N449K,D451G和G454S。其中所有毒株均发生V106I和S247N的变化。结论通过对HA和NA基因序列对比分析,该地区流感疫苗对当地居民有保护作用,但是毒株与疫苗株间发生相对抗原漂移,需要进一步观察毒株变异情况。
[Abstract]:Objective to study the gene characteristics and changes of influenza A virus (H1N1), and to provide the basis for the evaluation of influenza vaccine. Methods 15 isolates of H1N1 virus isolated from our hospital in June 2009, -2013, April were selected to reverse transcription of virus RNA. The whole gene of HA and NA of 15 strains of influenza A virus (H1N1) was amplified by PCR and sequenced and analyzed. The sequence was spliced with software Bioedit and MEGA, and the phylogenetic tree was plotted and the evolution tree was constructed by adjacent phase approach. Results 15 strains of H1N1 influenza A virus were both low pathogenic virus, sensitive to neuraminidase inhibitors and resistant to amantanes. The HA gene sequence of the experimental strain was homologous to the domestic and foreign strains. The variation degree of influenza virus antigen in 99.1%~99.7%. is large, and the strain HA of the strain has 14 amino acid sites, which are: A215E, D127E, E235K, E374K, G170E, H138R, L161I, I321V, L191I, P83S, eighty-third and 321st are the same as the domestic representative strains, but 15 are different from the foreign representative strains. A20V, N44S, V83M, V106I, E128G, H144Y, I188T, V241I, S247N, N248D, S334N, N369K, etc. It is necessary to further observe the variation of the virus relative to the antigen shift.

【作者单位】：哈励逊国际和平医院老年病二科;中国人民解放军白求恩国际和平医院;河南省第二人民医院药剂科;黑龙江省齐齐哈尔市第七医院传染一科;
【分类号】：R373

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