阴道毛滴虫病毒的鉴定及衣壳蛋白基因的原核表达

发布时间：2018-05-02 10:34

本文选题：阴道毛滴虫 + 病毒　；参考：《吉林大学》2006年硕士论文

【摘要】： 本研究首先从临床分离阴道毛滴虫,用肝浸汤进行纯培养,提取虫体总核酸,1%琼脂糖凝胶电泳,初步筛选出一株携带阴道毛滴虫病毒的虫株;其次通过电镜观察和生化分子生物学方法对病毒进一步进行鉴定,首次证实了在我国阴道毛滴虫体内存在病毒,核酸大小约为5.5kb,病毒为外观球形、直径33nm的dsRNA病毒,α-~(32)P标记的UTP测得该病毒具有RNA依赖RNA聚合酶活性,病毒序列与阴道毛滴虫病毒T1株序列具有82.9%的同源性;成功构建病毒衣壳蛋白基因的原核表达载体pET-cap2037,并在大肠杆菌BL(DE3)中进行了表达。本研究的结果为深入研究我国阴道毛滴虫病毒奠定了良好基础。
[Abstract]:In this study, we first isolated Trichomonas vaginalis from clinical practice, used liver dipping Soup for pure culture, extracted insect total nucleic acid, 1% agarose gel electrophoresis, preliminarily screened a strain of Trichomonas vaginalis virus. Secondly, the virus was identified by electron microscope observation and biochemical molecular biology method, and the first confirmation of vaginal dripping in our country was made. The virus has a virus in the body, the size of nucleic acid is about 5.5kb, the virus is a spherical dsRNA virus with a diameter of 33nm, and the UTP of alpha ~ (32) P marks that the virus has RNA dependent RNA polymerase activity. The sequence of the virus is homologous to the sequence of the T1 strain of Trichomonas vaginalis virus, and the prokaryotic expression vector of viral capsid protein gene is constructed. 037, and expressed in E. coli BL (DE3). The results of this study laid a good foundation for further study of Trichomonas vaginalis virus in China.

【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R383

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