大鼠面部慢性炎症痛导致三叉神经节表达P2X3神经元表型的转变

发布时间：2018-05-02 11:21

本文选题：痛觉过敏 + 触诱发痛　；参考：《华中科技大学》2006年硕士论文

【摘要】： 本研究试图探讨大鼠面部慢性炎症痛是否与三叉神经节内表达P2X受体亚型P2X3神经元细胞大小和表型变化有关。参照Neumann(1996)报道的研究方法,采用大鼠面部皮下注射松节油建立慢性炎症痛模型,用热测痛的方法测定面部皮肤的痛阈值,每天一次,连续测15天。用免疫组织化学技术观察大鼠面部慢性炎症后第5天三叉神经节内感觉神经元P2X3表达。采用体视学的方法测量表达P2X3神经节细胞大小及表型的变化。结果发现:炎症侧大鼠面部痛阈值与对照组相比明显降低,在第5天达到最低值,以后逐渐恢复,第13天开始痛阈恢复正常水平。炎症侧三叉神经节内表达P2X3神经元的平均细胞表面积(721±12μm2)与对照组(616±8μm2)相比明显增大(P0.01)。进一步观察发现表达P2X3小细胞群(950μm2)的表面积由炎症前的537±13μm2增加到炎症后的582±15μm2(P0.05)而且小细胞占总细胞的数量比例由炎症前的42.2±3.2%增加到炎症后的51.8±3.5%(P0.05);而表达P2X3受体的大细胞(950μm2)的数量比例由炎症前的6.5±1.9%增加到炎症后的12.8±2.2%(P0.05)。以上结果提示面部慢性炎症痛时,其三叉神经节内表达P2X3神经元的表型可发生改变,这可能与面部痛觉过敏和触诱发痛的形成有密切关系。由于支配牙髓的神经元为纯痛觉的细胞,因此该细胞可以作为研究痛觉传递的良好模型。本文介绍了一种支配牙髓的三叉神经节(TG)痛觉传入神经元的逆行追踪方法。用辣根过氧化物酶(HRP)注入牙髓腔内,通过逆向轴浆运输可以追踪到支配牙髓的TG痛觉传入神经元胞体。对其形态学特点进行分析,测量用图象分析仪测定了432个HRP追踪到的细胞面积的大小,并进行一定的统计分析。结果发现所有被HRP标记的三叉神经节细胞都是比较小的感觉神经元,其面积大小范围在100-350μm2之间。提示感受牙痛的初级感觉神经元主要为小细胞中比较小的神经元。这有助我们对对伤害性感受器的一些特点进行深入的研究和分析。
[Abstract]:This study attempts to investigate whether chronic inflammatory pain in the face of rats is related to the changes in cell size and phenotype of P2X receptor subtype P2X3 neurons expressed in the trigeminal ganglion. The chronic inflammatory pain model was established by subcutaneous injection of turpentine into the face of rats. The pain threshold of facial skin was measured by thermal pain test once a day for 15 days. The expression of P2X3 in sensory neurons of trigeminal ganglion was observed 5 days after chronic facial inflammation by immunohistochemical technique. The size and phenotype of ganglion cells expressing P2X3 were measured by stereology. The results showed that the threshold of facial pain in the inflammatory side of rats was significantly lower than that in the control group and reached the lowest level on the 5th day, then recovered gradually, and returned to the normal level on the 13th day. The average surface area of P2X3 neurons in the inflammatory trigeminal ganglion was 721 卤12 渭 m2), which was significantly larger than that in the control group (616 卤8 渭 m2). Further observation showed that the surface area of the expression of P2X3 was increased from 537 卤13 渭 m ~ 2 before inflammation to 582 卤15 渭 m ~ (2) P _ (0.05) after inflammation. The proportion of small cells to total cells increased from 42.2 卤3.2% before inflammation to 51.8 卤3.5 渭 m ~ (5) after inflammation, while the expression of P2X3 receptor increased from 42.2 卤3.2% to 51.8 卤3.5 渭 m ~ (0.05), while the expression of P2X3 receptor increased from 42.2 卤3.2% before inflammation to 51.8 卤3.5 渭 m ~ (5) after inflammation. The proportion of cytosol 950 渭 m 2) increased from 6.5 卤1.9% before inflammation to 12.8 卤2.2% after inflammation. These results suggest that the phenotypes of P2X3 neurons expressed in trigeminal ganglion may change in chronic inflammatory pain of the face, which may be closely related to the formation of facial hyperalgesia and touch-induced pain. As the neurons innervating the pulp are pure pain cells, this cell can be used as a good model for the study of pain transmission. A retrograde tracing method of pain afferent neurons of the trigeminal ganglion (TG) innervating the pulp is presented in this paper. Horseradish peroxidase (HRP) was injected into the pulp cavity, and the TG pain afferent neurons of the dental pulp could be traced by reverse axonal transport. The morphological characteristics of the cells were analyzed and the size of the cells tracked by 432 HRP were measured by image analyzer. The results showed that all the trigeminal ganglion cells labeled by HRP were relatively small sensory neurons, whose area ranged from 100-350 渭 m ~ 2 to 100 ~ 350 渭 m ~ 2. It is suggested that the primary sensory neurons that are sensitive to toothache are mainly small neurons in small cells. This will help us to study and analyze some of the characteristics of nociceptive receptors.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R363

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