结核分枝杆菌利福平依赖株和耐药株表观形态及分子生物学差异性分析

发布时间：2018-05-02 16:04

本文选题：结核分枝杆菌 + 利福平依赖菌　；参考：《中国病原生物学杂志》2014年07期

【摘要】：目的比较结核分枝杆菌利福平依赖株和耐药株在形态学及分子生物学方面的差异。方法制作利福平浓度梯度培养基,观察利福平依赖株和耐药株结核杆菌在含不同浓度利福平培养基上的菌落生长情况;采用抗酸染色和电镜观察二细菌的形态和超微结构;采用DNA测序技术分析细菌rpoB核心突变区的序列。结果利福平耐药菌株在200μg/ml利福平培养基上不生长,依赖株在700μg/ml药物培养基上生长旺盛。抗酸染色后电镜观察,利福平依赖菌株在含利福平培养基上生长良好,菌体粗大,细胞壁完整;对照培养基上的菌体细小,细胞壁破裂。测序分析二细菌在rpoB核心突变区均发生突变,其中依赖菌株的突变主要发生在531和526位,耐药株5个位点均有突变发生。结论利福平耐药株和依赖株结核分枝杆菌在形态学和超微结构上存在着明显差异,且这种差异可能与rpoB突变位点的不同存在一定相关性。
[Abstract]:Objective to compare the morphological and molecular biological differences between rifampicin dependent strains and drug resistant strains of Mycobacterium tuberculosis. Methods Rifampicin concentration gradient medium was prepared to observe the colony growth of rifampicin-dependent and drug-resistant strains of Mycobacterium tuberculosis on the medium containing rifampicin, acid fast staining and electron microscope were used to observe the morphology and ultrastructure of the two bacteria. DNA sequencing technique was used to analyze the sequence of the core mutation region of bacterial rpoB. Results Rifampicin resistant strains did not grow on 200 渭 g/ml rifampicin medium, but the dependent strains grew strongly on 700 渭 g/ml medium. After acid fast staining, we observed that rifampicin dependent strain grew well on rifampicin medium, the cell wall was large and the cell wall was intact, and the cell wall of the control medium was small and the cell wall was ruptured. Sequence analysis showed that the two bacteria mutated in the core mutation region of rpoB. The mutations of the dependent strains mainly occurred at the 531 and 526 loci, and the 5 loci of the resistant strains were all mutated. Conclusion there are obvious differences in morphology and ultrastructure between rifampicin-resistant and dependent strains of Mycobacterium tuberculosis, and this difference may be related to the different mutation sites of rpoB.
【作者单位】：河南省疾病预防控制中心;新乡市结核病防治所;
【分类号】：R378

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