不同成熟时期人卵母细胞冷冻保存的初步研究

发布时间：2018-05-09 15:50

本文选题：人卵母细胞 + 冷冻保存　；参考：《重庆医科大学》2006年硕士论文

【摘要】： 目的: 本研究通过对处于不同成熟时期卵子慢冻速融,应用偏振光显微镜(polarized light microscopy, Polscope)非侵入性的观察纺锤体的变化,以及复苏后卵子发育潜能研究,旨在为建立一种安全、有效的卵子冷冻保存与复苏方法提供理论依据和实验技术。方法: 不同成熟时期人卵母细胞采集自体外受精-胚胎移植(in vitro fertilization-embryo transfer, IVF-ET)和卵胞浆内单精子显微注射(intracytoplasmic sperm injection, ICSI)治疗周期的不育患者周期治疗中剩余的未成熟卵子(GV期和MI期)。实验分组:对照组(n=56),直接将未成熟卵子进行体外成熟培养(in vitro maturation, IVM),进行纺锤体观察,并行ICSI;MII卵冻融组(n=73),先将GV期和MI期卵子体外成熟培养到MII期进行冻融,将存活的卵子进行纺锤体观察,并行ICSI;MI/GV冻融组(n=91),将GV期和MI期卵子冷冻,冻融后复苏的卵子进行体外成熟培养,成熟的卵子进行纺锤体观察,并行ICSI。用慢冻速融的方法对成熟卵子和未成熟卵子进行冷冻和解冻,观
[Abstract]:Objective: The aim of this study was to establish a safe method by observing the changes of spindles and the developmental potential of oocytes after resuscitation by using polarized light microscope (polarizing microscope) to observe the changes of spindles during slow freezing and rapid melting of eggs at different maturation stages, and to study the developmental potential of oocytes after resuscitation. Effective methods of egg cryopreservation and resuscitation provide theoretical basis and experimental technique. Methods: Human oocytes were collected from IVF-ETS and intracytoplasmic sperm microinjection (ICSI) cycles of infertile patients at different maturation stages. The remaining immature oocytes were in the GV and MI phases in the cycles of intracytoplasmic sperm injection, ICSI). The experiment was divided into two groups: the control group (control group): immature eggs were cultured directly in vitro maturation, spindles observation, and ICSI-MII egg freezing and thawing group. GV and MI oocytes were cultured to MII for freeze-thawing in vitro. The surviving eggs were observed by spindles, and the ICSI-MIP / GV freeze-thawing group was used to freeze the eggs in GV and MI stages. The eggs recovered after freezing and thawing were cultured in vitro, the mature eggs were observed by the spindles, and the ICSI was performed. Freezing and thawing of mature and immature eggs by slow freezing and thawing.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R321-33

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