血管性眩晕症气虚血瘀型动物模型的建立

发布时间：2018-05-10 17:14

本文选题：血管性眩晕 + 椎-基底动脉供血不足　；参考：《湖南中医药大学》2005年硕士论文

【摘要】： 目的:建立一种操作简单、切实可行的具有病证结合特点的血管性眩晕症气虚血瘀型动物模型,并拟建立相对完备的行为、体征客观定量的评估体系。方法:选取健康日本大耳白兔16只(体重2.0±0.3kg/只),按体重由小到大编号,随机数字表法分成两组,其中造模组8只,对照组8只。首先将组织硬化剂注射至模型组家兔左侧颈椎横突软组织,造成左侧椎动脉狭窄、基底动脉及双侧椎动脉血流降低以建立慢性椎-基底动脉供血不足(VBI)模型;并在此基础上采用饥饿、高脂饮食复合因素制作出气虚血瘀证模型;然后将初步符合气虚血瘀证的动物选出,用旋转法诱发动物产生眩晕症。通过观察造模前后大耳白兔的行为、体征、体重、血液流变学、跳台耗时及脑血流图变化;比较模型组与正常组家兔脑干前庭神经核组织切片、肝脏DNA含量、跳台耗时;以跳台耗时作为评估眩晕程度和持续时间的指标。结果:1.与正常组比较,模型组脑干前庭神经核组织切片神经结构基本正常,并没有呈现急性缺血、缺氧样改变;旋转后跳台耗时明显延长,体征、体重、行为及肝脏DNA含量等气虚血瘀证量化评分增高。2.造模前后相比,模型动物的脑血流量明显下降,跳台耗时明显延长,血液流变学指标、行为表现、体重等发生改变。结论:该模型具有病证结合特点,不仅能够很好的反映眩晕程度及眩晕持续时间,而且能很好的反映中医气虚血瘀证候,可有效地应用于抗血管性眩晕中药的研究。经2005年度文献检索查新尚未发现国内外有相同的研究。
[Abstract]:Objective: to establish a simple and feasible animal model of vascular vertigo with Qi deficiency and blood stasis, and to establish an objective quantitative evaluation system of behavior and signs. Methods: sixteen Japanese white rabbits (weight 2.0 卤0.3kg/) were randomly divided into two groups according to their weight from small to large, including 8 model group and 8 control group. First, the tissue sclerosing agent was injected into the soft tissue of the left cervical transverse process in the model group, which resulted in the stenosis of the left vertebral artery and the decrease of blood flow of the basilar artery and bilateral vertebral artery to establish the model of chronic vertebrobasilar artery insufficiency. On this basis, the combined factors of hunger and high fat diet were used to make the model of Qi deficiency and blood stasis syndrome, and then the animals that accord with Qi deficiency and blood stasis syndrome were selected, and the animals were induced vertigo by rotation method. The changes of behavior, physical signs, body weight, hemorheology, platform time and cerebral blood flow graph were observed before and after modeling, and the brain stem vestibular nucleus sections, liver DNA content, time consuming of platform jumping were compared between the model group and the normal group. The time spent on the platform is used as an index to evaluate the degree and duration of vertigo. The result is 1: 1. Compared with the normal group, the nerve structure of the brain stem vestibular nucleus in the model group was basically normal, without acute ischemia and anoxic changes. The quantitative score of Qi deficiency and blood stasis syndrome, such as behavior and liver DNA content, was increased by 2. 2. Compared with those before and after modeling, the cerebral blood flow of the model animals decreased obviously, the time consuming of jumping platform was prolonged, the hemorheology index, the behavior performance and the weight of the model animals were changed. Conclusion: the model can not only reflect the degree of vertigo and the duration of vertigo, but also reflect the syndrome of qi deficiency and blood stasis, and can be effectively used in the study of anti-vascular vertigo. The same research has not been found at home and abroad by literature retrieval in 2005.
【学位授予单位】：湖南中医药大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R-332

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