体外培养的自体成纤维细胞血管内注射成活性研究

发布时间：2018-05-10 16:06

本文选题：血管畸形 + 介入治疗　；参考：《中国协和医科大学》2007年硕士论文

【摘要】： 【背景】头颈部血管畸形和／或血管瘤是小儿最常见的良性肿瘤之一，发病率为2.5-12.0％，约占所有软组织肿瘤的7％，全身血管瘤60％。头颈部血管畸形具有下列临床特点：1．部位表浅，临床症状发现早；2．病变严重程度与病程持续时间明显相关，应采取手段及早中断病程；3．治疗效果后的面貌外观对患者非常重要，要求创伤小，代价小。头颈部血管畸形的特点，决定了栓塞技术理应成为治疗血管畸形最好的方法，而目前的栓塞介入在头颈部血管畸形的治疗上处于被动或辅助地位，很大程度上与目前应用的各种栓塞剂的弊端有关，因此，只有栓塞剂的创新和变革，，才能使介入栓塞治疗在头颈部血管畸形的治疗中处于主动(没有明显症状时就给于干预治疗)和主导地位(充分发挥其创伤小的特点)。本课题组受到“动脉粥样硬化病变始于内皮损伤和成纤维细胞增生”这一假说的启发，提出了含自体成纤维细胞的生物栓塞剂在头颈部血管畸形介入治疗中的应用研究设想。【实验目的】探索取自皮肤组织的自体成纤维细胞体外培养、标记的方法以及血管内注射后的成活性，为应用含自体成纤维细胞的液体栓塞剂介入治疗头颈部血管畸形提供实验依据。【实验方法】取自SD大鼠的真皮组织通过组织贴块法体外培养原代成纤维细胞，在细胞传至第5代时，对细胞进行特殊标记(Brdu标记)后注射入大鼠血管内，一定时间后，通过血管切片、染色(Brdu，ki67，波形蛋白染色)，了解成纤维细胞的成活性和功能状态。【实验结果】组织贴块法体外培养原代成纤维细胞获得成功。血管切片、染色的结果证明，注射入血管内的成纤维细胞成活良好，分裂、增殖活跃，在注射后第5周血管腔几乎被成纤维细胞分泌的纤维组织封闭。【结论】含自体成纤维细胞的液体栓塞剂介入治疗血管畸形是可行的。
[Abstract]:[background] head and neck vascular malformation and / or hemangioma is one of the most common benign tumors in children. The incidence is 2.5-12.0, accounting for about 7 of all soft tissue tumors and 60 percent of systemic hemangioma. The head and neck vascular malformations have the following clinical features: 1. Superficial site, clinical symptoms found early 2. The severity of the disease was significantly correlated with the duration of the disease, and should be interrupted as early as possible. The appearance after treatment is very important for patients, requiring less trauma and less cost. The characteristics of head and neck vascular malformation determine that embolization should be the best way to treat vascular malformation, and the current embolization intervention is in a passive or auxiliary position in the treatment of head and neck vascular malformation. This is largely due to the drawbacks of the various embolic agents currently being used, so only the innovations and changes in the embolism, In order to make the interventional embolization in the treatment of head and neck vascular malformation in the initiative (no obvious symptoms are given to the intervention) and the dominant position (give full play to its small trauma characteristics). Inspired by the hypothesis that atherosclerotic lesions begin with endothelial injury and fibroblast proliferation, our research group proposed the application of biomembolic agents containing autologous fibroblasts in the interventional treatment of vascular malformations in the head and neck. [objective] to explore the in vitro culture, labeling and intravascular injection of autologous fibroblasts from skin tissue. To provide experimental evidence for interventional treatment of vascular malformation of head and neck with liquid embolic agent containing autologous fibroblasts. [methods] the primary fibroblasts were cultured in vitro from the dermal tissue of SD rats by tissue patch method. When the cells were transferred to the fifth passage, the cells were labeled with Brdu) and then injected into the blood vessels of the rats. After a certain time, the fibroblasts were injected into the blood vessels of the rats. The activity and function of fibroblasts were studied by means of vascular sections, Brduduki 67 and vimentin staining. The primary fibroblasts were successfully cultured in vitro by tissue patch method. The results showed that the fibroblasts injected into the blood vessels survived well, divided and proliferated actively. At the 5th week after injection, the vascular lumen was almost blocked by the fibrous tissue secreted by the fibroblasts. Conclusion it is feasible to treat vascular malformation with liquid embolic agent containing autologous fibroblasts.
【学位授予单位】：中国协和医科大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R329

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