超声介导微泡基因传递体系的研究

发布时间：2018-05-11 03:37

本文选题：超声 + 微泡造影剂　；参考：《化学进展》2014年08期

【摘要】：超声波能够在不损害人体正常组织的条件下,有效到达人体组织深处。超声造影剂(微泡),能够显著提高血液和组织的背向散射强度,广泛应用于心血管疾病的检测中。超声微泡介导基因转染技术是指通过微泡的空化作用,实现超声波能量在靶向组织处的富集和释放,进而促进此处组织细胞对基因药物的内化,达到高效转染的目的。由于具有无创、高效安全的特点,超声微泡介导在基因治疗中具有很大的优势和潜力。利用微泡的超声成像功能实现对病变组织诊断成像,利用靶向组织处的超声实现时间空间可控的目的基因的释放,利用微泡在超声作用下的"声孔效应",实现基因对多种生物膜的跨膜传递,达到基因药物的可视化传递与高效可控释放的目的。本文主要从超声造影剂的种类、超声微泡介导基因转染的机理、微泡基因传递体系的构建等方面,详细综述了这一领域最新的研究进展,并对超声微泡介导基因转染的发展进行了展望。
[Abstract]:Ultrasound can reach the depth of human tissues without harming normal tissues. Ultrasound contrast agent (microbubble) can significantly improve the blood and tissue backscatter intensity, widely used in the detection of cardiovascular disease. Ultrasound microbubble mediated gene transfection is to achieve the enrichment and release of ultrasonic energy in targeted tissues through the cavitation of microbubbles, thereby promoting the internalization of gene drugs in tissue cells and achieving the purpose of efficient transfection. Because of its noninvasive, high efficiency and safety characteristics, ultrasound microbubble mediated gene therapy has great advantages and potential. The ultrasonic imaging function of microbubbles was used to diagnose the pathological tissue and the target tissue was used to realize the release of the target gene in time and space. The transmembrane transfer of genes to various biofilms is realized by using the "acoustical hole effect" of microbubbles under the action of ultrasound, and the purpose of visual transmission of gene drugs and controlled release of gene drugs is achieved. In this paper, the latest research progress in the field of ultrasound contrast agent, the mechanism of ultrasound microbubble mediated gene transfection, the construction of microbubble gene transfer system and so on are reviewed in detail. The development of ultrasound microbubble mediated gene transfection was also prospected.
【作者单位】：浙江大学高分子科学与工程学系
【基金】：国家自然科学基金项目(No.51273177,81371571) 浙江省自然科学基金项目(No.LY13H180008)资助~~
【分类号】：R312

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