RAPD技术研究6种拟钉螺的遗传变异

发布时间：2018-05-11 02:42

本文选题：RAPD + 拟钉螺　；参考：《中国人兽共患病学报》2006年01期

【摘要】：目的对来自中国3省5地的6种拟钉螺进行遗传变异研究,明确它们的亲缘关系,为拟钉螺的DNA分类提供依据。方法采用随机扩增多态性DNA(RAPD)技术,对6种拟钉螺进行PCR扩增,根据扩增产物琼脂糖凝胶电泳的带型,计算各地拟钉螺的遗传距离,并绘制系统进化树;观察比较6种拟钉螺的外部形态和生态环境,并结合RAPD实验分类结果进行综合分析讨论。结果RAPD技术可将6种拟钉螺分为4类:①秉氏拟钉螺和向氏拟钉螺;②洪山拟钉螺和新店拟钉螺;③十堰拟钉螺;④武鸣拟钉螺。外部形态和生态环境方面,秉氏拟钉螺与向氏拟钉螺、洪山拟钉螺与新店拟钉螺的外部形态及生态环境较为相似,其它螺种间则差异较大。结论6种拟钉螺在基因水平上存在差异,此差异与拟钉螺外部形态、生态因素等传统分类因素相关联。
[Abstract]:Objective to study the genetic variation of 6 species of Oncomelania hupensis from 3 provinces and 5 regions in China, and to find out their genetic relationship, and to provide the basis for the classification of Oncomelania hupensis by DNA. Methods six species of Oncomelania hupensis were amplified by random amplified polymorphic DNA (RAPD) technique. According to the band patterns of agarose gel electrophoresis, the genetic distance of Oncomelania hupensis was calculated and the phylogenetic tree was plotted. The external morphology and ecological environment of six species of Oncomelania hupensis were observed and compared, and the results of RAPD experiment were analyzed and discussed. Results by RAPD technique, 6 species of Oncomelania hupensis could be divided into 4 groups: 1 species of Oncomelania hupensis, 1 species of Oncomelania hupensis, 2 species of Oncomelania hupensis, and 2 species of Oncomelania hupensis. In terms of external morphology and ecological environment, the external morphology and ecological environment of Oncomelania hupensis and Oncomelania angustii were similar, but the differences among other snails were significant. Conclusion there are genetic differences among the six species of Oncomelania hupensis, which are related to the traditional taxonomic factors such as external morphology and ecological factors of Oncomelania hupensis.
【作者单位】：华中科技大学同济医学院寄生虫学教研室华中科技大学同济医学院寄生虫学教研室福建省寄生虫病研究所湖北五峰县疾病控制中心
【基金】：湖北省自然科学基金资助项目(No.2005ABA174)
【分类号】：R383

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