抗人骨髓间充质干细胞单克隆抗体ZUC3在大鼠骨髓间充质干细胞生物学特性研究中的应用

发布时间：2018-05-15 12:45

  本文选题：大鼠 + 间充质干细胞　； 参考：《浙江大学》2006年硕士论文

【摘要】：间充质干细胞(mesenchymal stem cells,MSCs)因其自我更新、多向分化能力和低免疫原性,成为组织工程、再生医学的理想种子细胞,并在越来越多的临床疾病治疗中发挥作用。目前除人MSCs外,许多其他种属来源的MSCs也已有实验报道,如猫、狗、狒狒、兔、猪、山羊、绵羊等,但在动物模型研究中最常用的是大鼠。 作为MSCs鉴定的重要组成部分,其特异性的表面标记一直以来都是人们研究的热点,但至今尚未有何突破性的进展。通过单克隆抗体的筛选制备来寻找特异性表面分子是近年来开展的前沿技术。本课题小组通过该方法以人骨髓MSCs免疫小鼠,通过杂交瘤技术获得了稳定分泌抗人骨髓MSCs抗体的细胞株5株,分别将其分泌的抗体命名为ZUB1、ZUB4、ZUC3、ZUE12和ZUF10。在对5种抗体进行的特异性检测中发现,它们与除骨髓外的人体其他组织以及人骨髓中的其他细胞成分均不存在交叉反应,但ZUC3与大鼠和犬的骨髓MSCs存在明显的交叉反应,不具有种属特异性。 本研究旨在建立大鼠MSCs的体外培养与鉴定方法,分析ZUC3在大鼠MSCs检测鉴定中的价值,并应用ZUC3对处于不同生长阶段及向不同方向分化的大鼠MSCs作进一步检测,以了解ZUC3相应抗原在大鼠MSCs中的表达规律。研究分两部分:一、大鼠骨髓间充质干细胞的体外培养扩增和生物学特性研究,二、
[Abstract]:Mesenchymal stem cells (MSCs) have become ideal seed cells for tissue engineering and regenerative medicine because of their self-renewal, multi-directional differentiation and low immunogenicity, and play an important role in the treatment of more and more clinical diseases. At present, in addition to human MSCs, many other species of MSCs have been reported, such as cats, dogs, baboons, rabbits, pigs, goats, sheep and so on. As an important part of MSCs identification, its specific surface marker has been a hot research topic, but no breakthrough has been made so far. Screening and preparation of monoclonal antibodies to find specific surface molecules is a frontier technology developed in recent years. In this study, we immunized mice with human bone marrow MSCs and obtained 5 cell lines which secreted anti-human bone marrow MSCs antibody stably by hybridoma technique, and named ZUB1ZUB4ZUC3UC3UU12 and ZUF10respectively. In the specific detection of five antibodies, it was found that they did not cross react with other tissues except bone marrow and other cell components in human bone marrow, but ZUC3 had obvious cross reaction with rat and dog bone marrow MSCs. It has no species specificity. The purpose of this study was to establish an in vitro culture and identification method for rat MSCs, to analyze the value of ZUC3 in the detection and identification of rat MSCs, and to further detect rat MSCs in different growth stages and in different directions by ZUC3. To understand the expression of ZUC3 antigens in rat MSCs. The study is divided into two parts: first, the proliferation and biological characteristics of rat bone marrow mesenchymal stem cells in vitro.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R329.2

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