基质细胞衍生因子-1α和白细胞介素-1β诱导淋巴管内皮表型的作用

发布时间：2018-05-15 19:34

本文选题：基质细胞衍生因子-α + 白细胞介素-β　；参考：《解剖学报》2014年03期

【摘要】：目的探讨基质细胞衍生因子-1α(SDF-1α)及白细胞介素-1β(IL-1β)诱导内皮细胞表达淋巴管表型的作用。方法 SDF-1α和IL-1β分别诱导内皮细胞株CRL-1730,用Real-time PCR、Western blotting及免疫细胞化学等方法检测其内皮及淋巴管标志物,的表达情况。结果 SDF-1α诱导培养之后,CRL-1730细胞株的内皮细胞标志物血管性血友病因子(vWF)、血管内皮钙黏蛋白(VE-cadherin)、血管内皮生长因子受体(VEGFR)2随其浓度增高而表达降低,淋巴管标志物平足蛋白(podoplanin)、同源异形盒蛋白-1(Prox-1)和淋巴管内皮透明质酸受体-1(LYVE-1)随其浓度增高而表达增高。IL-1β诱导之后,CRL-1730细胞株的vWF、VEGFR2和podoplanin、prox-1、LYVE-1的变化趋势同SDF-1α,而VE-cadherin的表达量基本不变。结论 SDF-1α和IL-1β都能够诱导血管内皮细胞表达淋巴管标志物。
[Abstract]:Objective to investigate the expression of lymphatic phenotype in endothelial cells induced by stromal cell derived factor-1 伪 (SDF-1 伪) and interleukin-1 尾 (IL-1 尾). Methods Endothelial cell line CRL-1730 was induced by SDF-1 伪 and IL-1 尾, respectively. The expression of endothelial and lymphatic markers was detected by Real-time PCR Western blotting and immunocytochemistry. Results the expression of vascular Willebrand factor (VWF), vascular endothelial cadherin (VE-cadherin) and vascular endothelial growth factor receptor (VEGF) in CRL-1730 cell line decreased with the increase of SDF-1 伪 concentration. The expression of the lymphatic vessel marker podoplanin1, homologous cassette protein-1 Prox-1 and lymphatic endothelial hyaluronic acid receptor -1lYVE-1) was increased with the increase of its concentration. After induction of IL-1 尾, the changes of vWFFFVEGFR2 and podoplanprox-1 LYVE-1 in CRL-1730 cells were similar to those of SDF-1 伪, while the expression of VE-cadherin was almost unchanged. Conclusion both SDF-1 伪 and IL-1 尾 can induce vascular endothelial cells to express lymphatic markers.
【作者单位】：山东大学医学院解剖学与组织学胚胎学研究所;山东省郯城县第一人民医院肿瘤科;山东大学技术支持科;
【基金】：山东省自然科学基金资助项目(ZR2013HM096) 山东省医药卫生科技资助项目(2013WS0207)
【分类号】：R322.2

【参考文献】