机体氧化还原状态的研究

发布时间：2018-05-15 20:04

本文选题：氧化还原状态 + 谷胱甘肽　；参考：《四川大学》2005年博士论文

【摘要】：机体不断地遭受内源性和外源性活性氧(ROS)的攻击,但在机体丰富的抗氧化防御系统的作用下,机体的氧化-抗氧化间维持恒定的氧化还原状态,以维持机体的内环境稳定。机体的氧化-抗氧化能力间平衡失调则会引起机体REDST出现向氧化或还原方向进行偏移,当这种偏移超过一定限度则通过氧化还原敏感机制对机体产生氧化损伤或还原性损伤,引起各种各样疾病的产生。目前多从机体抗氧化物质的量的改变以及蛋白、脂质、核酸等遭受氧化损伤产生的一些代谢产物的增多等来对氧化损伤进行评价,尚无有效评价机体的REDST的方法。本实验即针对这些问题展开研究。鉴于GSH/GSSG是机体一种重要的氧化还原对,与NADPH、Vit C、Vit E、Trx以及其它相关酶等组成完整的抗氧化的氧化还原网络,着重讨论在生理以及不同疾病状态下血浆中GSH/GSSG、NADPH/NADP~+氧化还原状态的变化。首先,利用GSH、GSSG的生物化学特性,建立了一种新的测试条件对血浆中的GSH和GSSG进行荧光测试。利用DTT将GSSG还原为GSH,GSH的伯胺基和巯基与邻苯二甲醛(OPA)的两个醛基缩合成可发强荧光的物质异吲哚,从而运用荧光分光光度仪对血浆中的GSH以及GSSG进行定量。该方法准确,特异性高,回收较好,过程简捷。
[Abstract]:The organism is constantly attacked by endogenous and exogenous reactive oxygen species (Ros), but under the action of the body's abundant antioxidant defense system, the oxidation-oxidation phase of the organism maintains a constant redox state to maintain the stability of the body's internal environment. The imbalance between oxidation and oxidation resistance will cause the REDST to shift to the direction of oxidation or reduction, and when this deviation exceeds a certain limit, it will cause oxidative or reductive damage to the body through the mechanism of oxidation-reduction sensitivity. Cause various diseases. At present, the oxidative damage is evaluated from the changes of the amount of antioxidants and the increase of metabolites such as proteins, lipids, nucleic acids and so on. There is no effective method to evaluate the REDST of the body. This experiment aims at these problems. In view of the fact that GSH/GSSG is an important redox pair, a complete antioxidant redox network is formed with NADPHV Vit / Etrx and other related enzymes. The changes of GSH / GSSG / NADPH / NADP~ redox state in plasma under physiological and different disease conditions are discussed. Firstly, based on the biochemical characteristics of GSH-GSSG, a new test condition was established for the fluorescence detection of GSH and GSSG in plasma. Isoindole was synthesized by reducing GSSG to primary amine group and sulfhydryl group of GSH and phthalaldehyde OPA) by DTT, and then the GSH and GSSG in plasma were quantified by fluorescence spectrophotometer. The method is accurate, high specificity, good recovery and simple process.
【学位授予单位】：四川大学
【学位级别】：博士
【学位授予年份】：2005
【分类号】：R363

【引证文献】