大鼠巨细胞网状核和颈髓神经元纤维联系的实验研究

发布时间：2018-05-16 08:22

本文选题：网状结构 + 巨细胞网状核　；参考：《郑州大学》2007年硕士论文

【摘要】： 背景和目的脑干网状结构是中枢神经内的一个整合中心，从此中心不断发放冲动传导信息到大脑皮质、脊髓和小脑等其它脑区发挥调节作用。传统上网状结构被视作中枢神经内一个古老的、弥散的结构。其内除少数边界较清晰的核群外，似乎不易再划分其他核群：在功能上除参与内脏活动的调节(如呼吸、循环)外，看不出有什么重要作用。但进一步研究则提示脑干网状结构可能有许多更为重要和复杂的功能。关于它的结构，后来知道也不似原来想象的那么简单。随着荧光组织化学和免疫细胞化学方法的应用，许多特异神经递质的神经元在网状结构内被发现。但至今，对于网状结构还有许多问题尚待解决。中枢神经系统发挥功能的基础来源于其形态学结构。网状结构内其传出纤维主要投射到4个区域：丘脑、脊髓、小脑和脑干的其他结构。其作用于脊髓的纤维束对骨骼肌兴奋和抑制的调节有重要作用，从而调控了人肢体运动的随意和精确性。在网状结构向脊髓传出纤维进行调控的同时，脊髓的神经元亦投射纤维到网状结构内，参与了网状结构对运动神经元调控的反馈和控制。为进一步了解网状结构和脊髓神经元之间的纤维联系，为网状结构对运动的调控提供更多的理论依据，我们选取了网状结构内与运动调控密切相关的伸肌抑制区内的重要核团—巨细胞网状核(gigantocellular reticular nucleus，NGC)进行研究，探求它和颈段脊髓的纤维联系。方法 1、麦芽凝集素—辣根过氧化物酶的微量注射：选择成年大鼠，在大鼠右侧巨细胞网状核内微量注射麦芽凝集素—辣根过氧化物酶神经示踪剂。同时建立阴性对照组进行对照比较。 2、麦芽凝集素—辣根过氧化物酶的检测：注射48h后用TMB显色方法检测巨细胞网状核和颈段脊髓灰质内麦芽凝集素—辣根过氧化物酶的表达情况，并进行数据采集和分析。 3、海人酸的微量注射：同样在大鼠右侧巨细胞网状核内微量注射海人酸，造成对巨细胞网状核内神经纤维的伤害性刺激。 4、c-fos基因表达情况的检测：海人酸注入2h后检测实验大鼠颈段脊髓灰质内c-fos基因表达情况，以此推断巨细胞网状核和脊髓的纤维联系。结果 1、在巨细胞网状核内注射麦芽凝集素—辣根过氧化物酶，可在颈段脊髓灰质Ⅰ、Ⅱ板层发现顺行标记，在Ⅶ、Ⅷ板层发现逆行标记。 2、在巨细胞网状核内注入伤害性刺激物海人酸可以引起颈段脊髓灰质Ⅶ、Ⅷ板层内c-fos基因的显著性表达，与阴性对照组相比具有显著性差别，以α=0.05为检验水准，，两组之间的差别具有统计学意义。结论 1、通过对WGA-HRP示踪和c-fos基因表达情况的检测，说明以巨细胞网状核为主的脑干网状结构抑制区和颈段脊髓神经元之间存在直接的纤维联系。 2、从巨细胞网状核到脊髓的投射纤维多经由颈段脊髓灰质的Ⅰ、Ⅱ板层。而颈段脊髓灰质向巨细胞网状核的投射纤维多起源于Ⅶ、Ⅷ板层。
[Abstract]:Background and purpose
The brain stem reticular formation is an integration center in the central nervous system. From then on, impulse transmission information is continuously distributed to the cerebral cortex, and other brain regions, such as the spinal cord and the cerebellum, play an regulatory role. The traditional Internet structure is regarded as an old, diffuse structure in the central nerve. It seems not easy to be within a few clear nuclei. Further studies suggest that the brain stem reticular structure may have many more important and complex functions. The application of studies and immunocytochemical methods, many specific neurotransmitter neurons are found in the reticular formation. But there are still many problems still to be solved for reticular formation.
The basis of the function of the central nervous system is derived from its morphological structure. In the reticular structure its efferent fibers are projected mainly into 4 regions: the thalamus, spinal cord, cerebellum, and other brain stem structures. The fiber bundles acting on the spinal cord play an important role in the regulation of excitability and inhibition of skeletal muscles, thereby regulating the random and sperm movement of the human body. It is true that the neurons of the spinal cord also project the fibers into the reticular formation while the reticular formation of the spinal cord is transmitted to the spinal cord, and participates in the feedback and control of the reticular formation of the motor neurons.
In order to further understand the fiber connections between the reticular and spinal neurons, and to provide more theoretical basis for the regulation of the reticular formation of motion, we have selected the gigantocellular reticular nucleus (NGC), an important nucleus in the extensor inhibition zone, which is closely related to the regulation of motion. Explore the fibrous connection with the cervical spinal cord.
Method
1, microinjection of malt agglutinin - horseradish peroxidase: selected adult rats, microinjection of malt lectin to horseradish peroxidase nerve tracer in rat right giant cell reticular nucleus, and set up a negative control group for comparison.
2, detection of malt lectin - horseradish peroxidase: after injection of 48h, the expression of malt agglutinin - horseradish peroxidase in the giant cell reticular nucleus and cervical spinal cord gray matter was detected by TMB colorimetric method, and the data were collected and analyzed.
3, microinjection of sea human acid: also microinjection of sea human acid in the right giant cell reticular nucleus in the right side of the rat, causing injurious stimulation to the nerve fibers in the giant cell reticular nucleus.
4, detection of c-fos gene expression: the expression of c-fos gene in the gray matter of the cervical spinal cord was detected by sea human acid injected into 2H to infer the fibrous connection between the giant cell reticular nucleus and the spinal cord.
Result
1, the injection of malt lectin - horseradish peroxidase in the giant cell reticular nucleus could be found in the cervical spinal gray matter I and II lamina, and the retrograde labeling was found in the VII and VIII lamina.
2, injecting a nociceptive stimulator into the nucleus of the giant cell reticular nucleus could cause a significant expression of the c-fos gene in the gray matter VII and the VIII lamina of the cervical spinal cord. Compared with the negative control group, there was a significant difference. The difference between the two groups was statistically significant.
conclusion
1, through the detection of WGA-HRP tracer and c-fos gene expression, it is indicated that there is a direct fibrous connection between the brain stem reticular inhibition zone and the cervical spinal cord neurons based on the giant cell reticular nucleus.
2, the projective fibers from the giant cell reticular nucleus to the spinal cord are mostly through the cervical spinal gray matter I, II lamina, and the projection fibers from the gray matter of the cervical spinal cord to the giant cell reticular nucleus originate from the VII and the VIII lamina.

【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R329

【共引文献】