5-氮胞苷处理大鼠骨髓间充质干细胞与乳鼠心肌细胞共培养时的分化研究

发布时间：2018-05-18 15:38

本文选题：骨髓间充质干细胞 + 共培养　；参考：《中南大学》2006年博士论文

【摘要】：背景 CCMP是采用细胞移植对终末期心力衰竭进行心肌重建治疗的方法。胚胎心肌细胞、骨骼肌原细胞、胚胎干细胞、成纤维细胞、平滑肌细胞和造血干细胞都曾被用于CCMP。但是所有这些CCMP技术都有一定限制和缺陷，而且主要是在于用于移植的细胞类型。理想的用于CCMP的细胞应该具有以下特点：①多能的，，拥有在适当的刺激下分化为所需细胞类型的能力；②增殖能力必须有一定限度；③能够与宿主心肌形成功能整体。BMSCs就具有以上这些特点，它是多能的，在适当的刺激下可以分化为肌细胞、软骨细胞、成骨细胞、脂肪细胞，甚至心肌细胞，非常适合进行CCMP。 5-Aza是一种胞嘧啶核苷类似物，离体试验表明BMSCs在5-Aza的处理下可以分化为心肌细胞。目前在BMSCs移植治疗心肌损伤的在体动物研究中，一部分研究者在移植前先用5-Aza对使用的BMSCs进行处理，以促进BMSCs向心肌细胞的分化，另一部分研究者则不用5-Aza对BMSCs进行处理；5-Aza可用作单次处理，也有双次处理，是否用5-Aza处理以及处理次数对于BMSCs向心肌细胞分化的影响，目前尚无相关的文献报告。Fukuhara等人的研究表明，小鼠BMSCs与乳鼠心肌细胞共培养能够模拟心脏细胞移植时的环境。本研究的目的在于利用与乳鼠心肌细胞共培养模型，拟探讨用5-Aza处理能否促进BMSCs向心肌细胞的分化以及处理次数对分化的影响。
[Abstract]:Background CCMP is a method of myocardial reconstruction for end-stage heart failure by cell transplantation. Embryonic cardiomyocytes, skeletal muscle cells, embryonic stem cells, fibroblasts, smooth muscle cells and hematopoietic stem cells have all been used in CCMPs. However, all of these CCMP techniques have limitations and drawbacks, and are mainly related to the cell types used for transplantation. The ideal cells for CCMP should have the following characteristics: 1: 1 pluripotent, and the ability to differentiate into the desired cell type under appropriate stimulation must be limited; 3. BMSCs have these characteristics as a whole. BMSCs can differentiate into myocytes, chondrocytes, osteoblasts, adipocytes and even cardiomyocytes under proper stimulation, which is suitable for CCMPs. 5-Aza is a cytosine nucleoside analogue. In vitro, BMSCs can differentiate into cardiomyocytes under the treatment of 5-Aza. At present, in the study of BMSCs transplantation in vivo for the treatment of myocardial injury, some researchers used 5-Aza to treat the BMSCs before transplantation, in order to promote the differentiation of BMSCs into cardiomyocytes. Another group of researchers did not use 5-Aza to treat BMSCs. 5-Aza could be used as a single or double treatment. Whether or not 5-Aza was used and how many times of treatment affected the differentiation of BMSCs into cardiomyocytes. At present, there is no related literature report. Fukuhara et al., shows that co-culture of mouse BMSCs and neonatal rat cardiomyocytes can simulate the environment of heart cell transplantation. The purpose of this study was to investigate whether 5-Aza treatment could promote the differentiation of BMSCs into cardiomyocytes and the effect of the number of treatments on the differentiation by using co-culture model with neonatal rat cardiomyocytes.
【学位授予单位】：中南大学
【学位级别】：博士
【学位授予年份】：2006
【分类号】：R329.2

【共引文献】