脊髓NMDA受体在大鼠慢性内脏高敏机制中的作用

发布时间：2018-05-19 17:53

本文选题：SD大鼠 + 慢性内脏高敏　；参考：《福建医科大学》2007年硕士论文

【摘要】： 目的 1.建立大鼠慢性内脏高敏感模型。 2.观察脊髓NMDA受体与慢性内脏高敏感的关系,探讨慢性内脏高敏感的可能机制。方法研究选用SD大鼠,模型组大鼠在出生后第8-15天内,每天接受一次结直肠扩张刺激;对照组大鼠在出生后8-15天,除了不行结直肠扩张刺激外,其他情况同模型组。大鼠成年后用腹部回缩反射(AWR),进行肠道敏感性评估。模型组和对照组通过鞘内注射方式,分别给予NMDA受体阻断剂MK-801和人工脑脊液,观察各组之间腹外斜肌放电有否存在差异;同时运用免疫组化法观察NMDA-NR2B受体的在各组脊髓中表达及分布情况。结果 1.模型成功的评价方式①采用AWR评分评价内脏敏化程度在20～60mmHg CRD时,模型组AWR评分显著高于对照组(P0.05),而在80mmHg CRD时两组评分差异不明显;对照组痛阈为30.418±1.34mmHg,模型组痛阈为13.125±1.34mmHg(P0.001)。②腹外斜肌放电测量评价内脏敏化程度在20～60mmHg CRD时,模型组腹外斜肌放电明显高于对照组(P0.05);两组痛阈分别为:对照组37.5±4.53mmHg,模型组25.0±3.27mmHg(P0.05)。 2.鞘内注射给予NMDA受体阻断剂—MK-801(1.5nmol/10μl)后,模型组腹外斜肌对CRD反应较给药前显著(P0.05),痛阈显著升高(P0.05);而对照组腹外斜肌对CRD反应较给药前无明显改变,痛阈变化不显著。 3. NMDAR-2B在相应脊髓节段中表达模型组与对照组大鼠在脊髓相应节段背角各层中可见数量不等的NMDAR-2B阳性细胞,多为圆形或椭圆形的神经元,胞浆着色呈棕黄色。图像分析结果显示,模型人工脑脊液组较对照组显著升高(P0.01);但给予MK-801后,模型组表达降低(P0.05)。结论 1.模型组大鼠存在慢性内脏高敏的表现。 2.AWR和腹外斜肌放电测量两种方法均可用于评价大鼠内脏高敏状态。 3.大鼠脊髓NMDA受体可能参与慢性肠道高敏感机制。
[Abstract]:Purpose 1. The chronic visceral hypersensitivity model of rats was established. 2. To investigate the relationship between NMDA receptor of spinal cord and chronic visceral hypersensitivity and to explore the possible mechanism of chronic visceral hypersensitivity. Method Sprague-Dawley rats were used in the study. Rats in the model group received colorectal dilatation once a day within 8-15 days after birth, while in the control group, the other conditions were the same as those in the model group except that the rats in the control group were not stimulated by colorectal dilatation at 8-15 days after birth. The intestinal sensitivity was evaluated by abdominal reflex AWRRV in adult rats. The model group and the control group were given NMDA receptor blocker MK-801 and artificial cerebrospinal fluid respectively by intrathecal injection. Immunohistochemical method was used to observe the expression and distribution of NMDA-NR2B receptor in the spinal cord. Result 1. Model successful Evaluation Mode 1: when the degree of visceral sensitization was evaluated by AWR score, the AWR score of model group was significantly higher than that of control group (P 0.05), but there was no significant difference between the two groups in 80mmHg CRD. The pain threshold of the control group was 30.418 卤1.34 mmHg, the pain threshold of the model group was 13.125 卤1.34mmHg(P0.001).2, and the degree of visceral sensitization was evaluated when the pain threshold of the model group was 13.125 卤1.34mmHg(P0.001).2. At the time of 20~60mmHg CRD, the pain threshold of the model group was significantly higher than that of the control group (P 0.05), and the pain threshold of the two groups was 37.5 卤4.53 mm Hg in the control group and 25.0 卤3.27 mmHg in the model group. 2. After intrathecal injection of NMDA receptor blocker -MK-801 (1.5 nmol / 10 渭 l), the response of the external oblique muscle to CRD in the model group was significantly higher than that before administration of the drug, and the pain threshold was significantly increased, while the response of the oblique abdominal muscle to CRD was not significantly changed and the pain threshold was not significantly changed in the control group. 3. The expression of NMDAR-2B in the corresponding spinal cord segment of the model group and the control group showed different numbers of NMDAR-2B positive cells in each layer of the dorsal horn of the corresponding segment of spinal cord, most of which were round or elliptical neurons, and the cytoplasm was brownish yellow. The results of image analysis showed that the expression of P0. 01 in the model group was significantly higher than that in the control group, but the expression of P0. 05 in the model group was decreased after MK-801 administration. Conclusion 1. Chronic visceral Gao Min was found in the model group. 2.AWR and external oblique muscle discharges can be used to evaluate visceral Gao Min in rats. 3. The NMDA receptor of spinal cord in rats may be involved in the mechanism of chronic intestinal hypersensitivity.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R363

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