输注人PBMC建立“人-鼠”异种移植物抗宿主病模型1

发布时间：2018-05-25 17:05

本文选题：PBMC + 器官移植　；参考：《中国免疫学杂志》2014年12期

【摘要】：目的:摸索X-GVHD模型的合适小鼠品系,建立稳定的"人-鼠"X-GVHD模型。方法:选取了裸鼠、NOD/SCID经亚致死剂量γ射线全身照射后,腹腔输注健康人外周血单核细胞(PBMC)建立异种X-GVHD模型。通过检测人T细胞在模型鼠外周血、组织、器官中的浸润情况等指标(采用流式细胞术和免疫组化技术),比较两种模型鼠中人免疫细胞的浸润率和各组生存时间,从而确定建立X-GVHD模型的合适小鼠品系,并摸索人PBMC的输注途径和合适细胞量,以及观察免疫细胞表型变化与功能的最佳时间窗口。结果:NOD/SCID鼠更适合诱导"人-鼠"X-GVHD模型的小鼠;腹腔输注途径和尾静脉输注途径对建立"人-鼠"X-GVHD模型无明显差异;腹腔输注5×107以上人PBMC可以成功建立"人-鼠"X-GVHD模型。在采用优化后的实验条件建立模型中,监测人T细胞表型动态变化与功能的最佳时间窗口为7~11 d;模型鼠的平均生存时间为(14.16±1.77)d。结论:NOD/SCID鼠通过腹腔注射5×107以上人PBMC可以成功建立"人-鼠"X-GVHD模型,7~11 d为监测人T细胞表型动态变化与功能的最佳时间。
[Abstract]:Objective: to explore the suitable strain of X-GVHD model and to establish a stable human-mouse X-GVHD model. Methods: the xenogeneic X-GVHD model was established in nude mice after whole body irradiation with sublethal dose 纬-ray and intraperitoneal infusion of PBMCs from peripheral blood monocytes of healthy people. The infiltration rate and survival time of human T cells in peripheral blood, tissues and organs of model rats were compared by flow cytometry and immunohistochemical technique. Thus, the appropriate strain of X-GVHD model was established, and the infusion pathway and appropriate cell quantity of human PBMC were explored, and the best time window for observing the phenotypic changes and functions of immune cells was also found. Results the mice with "human-mouse" X-GVHD model were more suitable to be induced by the mice than the control group, and there was no significant difference between the intraperitoneal infusion pathway and the caudal vein infusion pathway in the establishment of the "human-mouse" X-GVHD model, and the "human-mouse" X-GVHD model could be successfully established by intraperitoneal infusion of more than 5 脳 10 ~ 7 human PBMC. The optimal time window for monitoring the phenotypic changes and functions of human T cells was 711 days, and the average survival time of model mice was 14.16 卤1.77 days. Conclusion the "human-mouse" X-GVHD model can be successfully established by intraperitoneal injection of 5 脳 10 ~ (7) human PBMC into the control group. The best time to monitor the dynamic changes and function of human T cell phenotypes is to establish the "human-mouse" X-GVHD model for 7 ~ 11 days.
【作者单位】：湖北医药学院附属东风医院;武汉科技大学医学院;华中科技大学同济医学院免疫学系;
【基金】：国家自然科学基金资助项目(30972697;30901320)
【分类号】：R392

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