抗原表位肽的快速筛选鉴定及其在免疫检测和抗体制备中的应用

发布时间：2018-05-25 22:37

本文选题：抗原表位 + 预测　；参考：《第四军医大学》2005年博士论文

【摘要】：抗原表位是抗原的核心部分,目前确定抗原表位的常用方法有:酶促或化学裂解片段分析法、蛋白印迹法、肽扫描、化学修饰或突变分析、表面呈现肽库和随机片段表达库筛选,以及X射线衍射和核磁共振(NMR)分析等,这些方法大多操作繁琐、难度较大,效率非常低。随着计算机技术在生物技术领域的应用和发展,研究发现,抗原表位与蛋白质的氨基酸序列的结构特征存在特定的关系,从而建立了一系列基于这些结构特征参数的抗原表位预测分析方案,预测抗原表位的正确率可以达到75%以上。在筛选鉴定抗原表位时,如果先通过抗原表位的预测使待选表位的范围大大缩小,再用相应的实验来鉴定,往往可取得事半功倍的效果。
[Abstract]:Antigenic epitopes are the core of antigens. At present, the commonly used methods for the determination of antigen epitopes are enzymatic or chemical cleavage fragment analysis, Western blotting, peptide scanning, chemical modification or mutation analysis. Surface presentation peptide library and random fragment expression library screening, X-ray diffraction and nuclear magnetic resonance (NMR) analysis, most of these methods are cumbersome, difficult and inefficient. With the application and development of computer technology in the field of biotechnology, it has been found that there is a specific relationship between antigenic epitopes and the structural characteristics of amino acid sequences of proteins. A series of antigenic epitope prediction and analysis schemes based on these structural characteristic parameters are established, and the accuracy of antigen epitope prediction can reach more than 75%. In the screening and identification of antigen epitopes, if the range of epitopes to be selected is greatly reduced by the prediction of antigen epitopes, and then identified by corresponding experiments, the results can be doubled with half the effort.
【学位授予单位】：第四军医大学
【学位级别】：博士
【学位授予年份】：2005
【分类号】：R392.1

【引证文献】