DHEA对小鼠脾淋巴细胞活化的调节

发布时间：2018-05-26 06:05

本文选题：脱氢表雄酮 + 淋巴细胞活化　；参考：《天津医科大学》2005年硕士论文

【摘要】：目的研究DHEA对未活化及活化小鼠脾细胞增生、体内抗体生成、脾细胞细胞因子IL-2、IL-4、IL-10和TGF-β mRNA表达水平,以及辅助刺激分子CTLA-4、B7-1、B7-2和凋亡因子Bax和Bcl-2mRNA表达水平。探讨DHEA对于不同活化状态的淋巴细胞功能的调节。方法采用ELISA方法检测人血清免疫小鼠血清中IgG的产生情况;采用MTT法检测DHEA对conA活化的小鼠正常及致敏脾细胞增生的调节;采用RT-PCR方法测定脾细胞细胞因子IL-2、IL-4、IL-10、TGF-β和辅助刺激分子CTLA-4、B7-1、B7-2、B7h、ICOS以及凋亡因子BAX和Bcl-2mRNA表达水平。结果在人血清免疫小鼠,后给药组小鼠抗体水平显著高于未给药组(p0.05),且先给药组小鼠抗体水平明显高于后给药组和未给药组(p0.01,p0.01)。在0.5μ～5.0μg%qml的范围内,DHEA对未活化的脾细胞无明显的细胞毒性作用,也无明显的活化作用。在DHEA浓度为0.5μg/ml和1.0μg%qml时,对不同浓度ConA活化脾细胞的增生均无明显的调节作用(p0.05)。仅在DHEA浓度为5.0μg/ml、ConA浓度为5.0μg/ml时,DHEA对活化脾细胞增生表现出抑制作用(p0.05)。 DHEA可显著性下调培养未活化脾细胞IL-2 mRNA水平(P0.01)。活化脾细胞的IL-2 mRNA水平显著升高,DHEA同样能够显著下调活化脾细胞IL-2mRNA水平(P0.05)。DHEA可显著性下调未活化脾细胞IL-4mRNA水平(p0.01)。活化脾细胞的IL-4 mRNA水平显著升高,DHEA同样能够显著下调活化脾细胞IL-4 mRNA水平(p0.01)。DHEA可下调培养24小时的脾细胞IL-10 mRNA水平(P0.01),但对培养48小时的脾细胞IL-10基因的转录无显著的调节作用(P0.05)。未能观察到DHEA对TGF-β基因转录的调节作用。
[Abstract]:Objective to study the effects of DHEA on the proliferation of splenocytes, the production of antibodies, the expression of IL-10 and TGF- 尾 mRNA, the expression of CTLA-4nB7-1B7-2 and apoptotic factor Bax and Bcl-2mRNA in unactivated and activated mice. To investigate the regulation of DHEA on lymphocyte function in different activated states. Methods ELISA method was used to detect the production of IgG in serum of mice immunized with human serum, and MTT method was used to detect the regulation of DHEA on the proliferation of normal and sensitized splenocytes in mice activated by conA. The expression of IL-2mIL-4 IL-10 TGF- 尾 and CTLA-4B7-1, B7-2H7, BAX and Bcl-2mRNA were measured by RT-PCR method. The expression of IL-10 TGF- 尾 and CTLA-4B7-1, B7-B7-2H7, TGF- 尾, TGF- 尾, TGF- 尾 were measured by RT-PCR. Results the level of antibody in mice immunized with human serum was significantly higher than that in the control group (P 0.05), and the antibody level in the first administration group was significantly higher than that in the later administration group and the non-administration group. In the range of 0. 5 渭 g%qml, DHEA had no obvious cytotoxicity or activation to the unactivated splenocytes. When the concentration of DHEA was 0. 5 渭 g/ml and 1. 0 渭 g%qml, the proliferation of splenocytes activated by ConA at different concentrations had no obvious effect on p0. 05%. Only when the concentration of DHEA was 5.0 渭 g 路ml ~ (-1) Cona was 5.0 渭 g/ml. DHEA significantly down-regulated the level of IL-2 mRNA in cultured unactivated splenocytes (P0.01). The level of IL-2 mRNA in activated splenocytes was significantly increased and the level of IL-2mRNA in activated splenocytes was also significantly down-regulated. DHEA could significantly down-regulate the IL-4mRNA level of unactivated splenocytes. The level of IL-4 mRNA in activated splenocytes was significantly increased. DHEA could also significantly down-regulate the level of IL-4 mRNA of activated splenocytes. DHEA could down-regulate the level of IL-10 mRNA of splenocytes cultured for 24 hours, but had no significant effect on the transcription of IL-10 gene of splenocytes cultured for 48 hours. The regulation of TGF- 尾 gene transcription by DHEA was not observed.
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R392

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