新发现甲状腺胃动素的表达及其临床意义研究

发布时间：2018-05-26 13:44

本文选题：胃动素 + 甲状腺　；参考：《青岛大学》2006年硕士论文

【摘要】：目的:探讨胃动素(Motilin,MTL)前体mRNA基因在甲状腺组织中的表达及序列测定;观察MTL活性肽在甲状腺的定性表达和分布;检测MTL及其mRNA在甲状腺不同病变中的表达和临床意义。方法:采用RT-PCR、Southern Blot杂交和分子克隆等先进分子生物学技术,克隆并测定人甲状腺MTL前体mRNA基因序列;采用荧光免疫组织化学双染技术,观察胃动素免疫活性物(MTL-IR)在甲状腺组织中的分布和定性表达;采用实时荧光定量PCR(Real Time PCT,RT-PCR)和荧光免疫组化技术,比较MTL前体mRNA和MTL活性肽在正常甲状腺和甲状腺良、恶性肿瘤组织中表达。结果:(1)RT-PCR及Southern Blot杂交结果首次报道,人甲状腺组织有MTL前体mRNA的表达。MTL mRNA分子序列测定结果显示,人甲状腺组织MTL mRNA基因序列与美国基因库报道的人小肠MTL mRNA基因序列完全相同。(2)免疫荧光组化研究显示,甲状腺组织内有大量MTL免疫阳性细胞的表达,该细胞主要分布在甲状腺中央区域,散在于滤泡间或滤泡上皮之间,且MTL与降钙素(Calcitonin,CT)共存,提示MTL表达于甲状腺C细胞。(3)甲状腺肿瘤组织内有MTL免疫阳性物的表达,MTL免疫阳性细胞或弥散(髓样癌)或集中(滤泡癌、乳头癌、甲状腺嗜酸性腺瘤)分布;甲状腺髓样癌和嗜酸性腺瘤的MTL表达明显高于正常甲状腺组织(P0.001);但与正常甲状腺组织相比甲状腺乳头状癌和滤泡癌MTL的表达明显降低(P0.01);结节性甲状腺肿与正常甲状腺组织相比MTL的表达无显著差异(P0.05)。(4)甲状腺肿瘤组织内有MTL前体mRNA的表达。甲状腺髓样癌和嗜酸性腺瘤的MTL前体mRNA的表达明显高于正常甲状腺组织(P0.01);但与正常甲状腺组织相比甲状腺乳头状癌和滤泡癌MTL前体mRNA的表达明显降低(P0.05);结节性甲状腺肿与正常甲状腺组织相比MTLmRNA的表达无显著差异(P0.05)。结论:人甲状腺组织有MTL前体mRNA的表达,且人甲状腺组织MTL mRNA
[Abstract]:Objective: to investigate the expression and sequence determination of mRNA gene precursor of motilin motilinlus (MTL) in thyroid tissue, to observe the qualitative expression and distribution of MTL active peptide in thyroid gland, and to detect the expression and clinical significance of MTL and mRNA in different thyroid lesions. Methods: the precursor mRNA gene of human thyroid MTL was cloned and sequenced by RT-PCR Southern Blot hybridization and molecular cloning. To observe the distribution and qualitative expression of motilin immunoreactive substance (MTL-IRR) in thyroid tissue, to compare MTL precursor mRNA and MTL active peptide in normal thyroid and thyroid benign by real-time fluorescence quantitative PCR(Real Time PCT RT-PCR and fluorescence immunohistochemical technique. It is expressed in malignant tumor tissues. Results the results of RT-PCR and Southern Blot hybridization showed for the first time that MTL precursor mRNA was expressed in human thyroid tissue. The MTL mRNA gene sequence of human thyroid tissue was exactly the same as that of human small intestine MTL mRNA gene reported by American gene bank. The immunohistochemical study showed that a large number of MTL immunoreactive cells were expressed in thyroid tissue. The cells were mainly distributed in the central thyroid gland, scattered between follicles or between follicular epithelium, and MTL co-existed with calcitonin C +. It is suggested that the expression of MTL immunoreactive cells in thyroid neoplasms is expressed in thyroid C cells. 3) the distribution of MTL immunoreactive cells in thyroid neoplasms is diffuse (medullary carcinoma) or concentrated (follicular carcinoma, papillary carcinoma, thyroid eosinophilic adenoma). The expression of MTL in medullary thyroid carcinoma and eosinophilic adenoma was significantly higher than that in normal thyroid tissue (P 0.001), but the expression of MTL in thyroid papillary carcinoma and follicular carcinoma was significantly lower than that in normal thyroid tissue, and the expression of MTL in nodular goiter was significantly lower than that in normal thyroid tissue. There was no significant difference in the expression of MTL precursor mRNA in thyroid neoplasms compared with the expression of MTL. The expression of MTL precursor mRNA in medullary thyroid carcinoma and eosinophilic adenoma was significantly higher than that in normal thyroid tissue, but the expression of MTL precursor mRNA in thyroid papillary carcinoma and follicular carcinoma was significantly lower than that in normal thyroid tissue. There was no significant difference in the expression of MTLmRNA between adenoma and normal thyroid tissue. Conclusion: there is MTL precursor mRNA expression in human thyroid tissue and MTL mRNA in human thyroid tissue.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R341

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