高糖饲料对大鼠糖脂代谢及肝脏代谢综合征相关基因2表达的影响

发布时间：2018-05-26 19:55

本文选题：高糖饲料 + 肝脏　；参考：《四川大学》2006年硕士论文

【摘要】：近年来的研究发现，代谢综合征(metabolic syndrome，MS)是动脉粥样硬化(atherosclerosis，AS)的一种新的危险因子。研究表明MS的基本代谢特征是糖、脂代谢异常，但这些糖、脂代谢异常改变发生的机制以及在MS和AS的发生发展中所起作用尚未阐明。本研究室曾采用高糖饲料喂养大鼠建立了一种动物模型，该模型的基本特点是空腹血糖、甘油三酯(total triglyceride，TG)升高；并呈现明显高凝状态，明显具有MS的一些基本特征。将该模型大鼠肝脏mRNA与普通饲料喂养大鼠肝脏mRNA消减杂交，建立了该模型的肝脏差异表达基因cDNA文库，并对文库进行筛选，，找到了一些差异表达序列标签(expressed sequence tag，EST)。将EST与GenBank比对后寻找到与其中一条EST同源的大鼠基因，因为国内外尚无该基因结构和功能的报道。我们暂时将该基因命名为代谢综合征相关基因2(metabolic syndrome relative gene 2，MSRG2)。本研究在过去工作基础上进一步探讨高糖饲料对大鼠血糖、血脂、肝脏脂质，特别是血浆及肝脏磷脂及其分布的影响，并测定高糖饲料对大鼠肝脏MSRG2表达的影响。平衡饲料喂养1周后，实验组大鼠喂饲高糖饲料，对照组喂饲普通饲
[Abstract]:In recent years, metabolic syndrome (MS) has been found to be a new risk factor for atherosclerotic atherosclerosis (ASA). The results showed that the basic metabolic characteristics of MS were abnormal metabolism of sugar and lipid, but the mechanism of the abnormal metabolism of sugar and lipid and its role in the occurrence and development of MS and as had not been clarified. An animal model was established by feeding rats with high sugar diet in our laboratory. The basic characteristics of the model were fasting blood glucose, total triglyceride-TG), and showed obvious hypercoagulability and had some basic characteristics of MS. The subtractive hybridization of mRNA in the liver of the model rat and the normal feed fed rat liver was used to establish the cDNA library of the liver differential expression gene. The library was screened and some differentially expressed sequence tagESTs were found. After comparing EST with GenBank, a rat gene homologous to one of EST was found, because there are no reports on the structure and function of this gene at home and abroad. We temporarily named the gene 2(metabolic syndrome relative gene 2 MSRG 2. In this study, the effects of high glucose diet on blood glucose, blood lipid, liver lipids, especially plasma and liver phospholipid and their distribution in rats were further studied, and the effects of high glucose diet on the expression of MSRG2 in rat liver were determined. After a week of balanced diet, rats in the experimental group were fed with high-sugar diet, while the control group was fed with common feed.
【学位授予单位】：四川大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R363

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