白念珠菌基因芯片的制备和应用及两个新基因的克隆和功能鉴定

发布时间：2018-05-29 01:27

本文选题：白念珠菌 + 基因芯片　；参考：《第二军医大学》2005年博士论文

【摘要】：白念珠菌是临床免疫功能低下患者最为常见的致病真菌,常造成粘膜和深部真菌感染。我们利用SMART技术快速构建了白念珠菌高比例全长cDNA文库,并制备了含有3096个unigene的白念珠菌cDNA微阵列芯片。利用所构建的基因芯片我们研究了共六对氟康唑敏感和耐药白念珠菌配对菌株的大规模基因表达谱,证实不同环境下产生的白念珠菌耐药性均与多药耐药基因CDR的mRNA表达增加有关,并从中鉴定了一些与药物耐受性相关的共同差异表达基因和一些有趣的功能连锁基因群。同时,通过比较耐药和敏感菌株之间的差异,我们从大量差异表达基因中筛选出两个耐药相关新基因,在详尽生物信息学分析的基础上,获得新基因的完整编码序列并从敏感性,外转运,细胞周期,及形态毒力等几方面对其功能进行了系统研究。
[Abstract]:Candida albicans is the most common pathogenic fungus in patients with low immune function, often causing mucosal and deep fungal infection. We rapidly constructed a full-length cDNA library of Candida albicans by using SMART technique and fabricated a cDNA microarray of Candida albicans containing 3096 unigene. Using the constructed gene chip we studied the large-scale gene expression profiles of six pairs of fluconazole-sensitive and drug-resistant Candida albicans strains. The results showed that the drug resistance of Candida albicans in different environments was related to the increase of mRNA expression of multidrug resistance gene CDR, and some common differentially expressed genes related to drug tolerance and some interesting functional linkage gene groups were identified. At the same time, by comparing the differences between drug resistance and sensitive strains, we screened two new genes related to drug resistance from a large number of differentially expressed genes, which were based on detailed bioinformatics analysis. The complete coding sequence of the new gene was obtained and its function was systematically studied from the aspects of sensitivity, external transport, cell cycle and morphologic virulence.
【学位授予单位】：第二军医大学
【学位级别】：博士
【学位授予年份】：2005
【分类号】：R346

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