长期低硒低碘对仔三、四代发育期大鼠抗氧化酶及脑中单胺类神经递质的影响

发布时间：2018-05-29 02:17

  本文选题：硒 + 碘　； 参考：《汕头大学》2005年硕士论文

【摘要】：背景与目的 硒是人和动物生命活动所必需的微量元素。硒缺乏会引起含硒酶活性降低, 氧自由基清除受阻、内分泌紊乱等一系列机体功能障碍,其对神经系统的影响也 越来越引起学者们的重视。硒缺乏与碘缺乏密切相关,低碘地区常同时伴有低 硒。在政府二十多年的全民补碘后,碘缺乏所致的典型的克汀病已基本得到控制, 但是仍有许多亚临床克汀病弱智儿童出现,故认为硒可能是亚克汀病的一个重要 的碘外因素。 既往硒、碘缺乏的动物实验研究多集中于原代及仔一代,且多为成年期。为 模拟硒碘缺乏病区人口世代生存繁衍于低硒或联合低碘环境所致的长期损害,尤 其是对子代儿童所致的智力损害,我们与多方学者共同合作建立了人工膳食低硒 低碘喂养繁殖的SD大鼠动物模型,并历时近两年繁衍至仔四代。本模型仔三代 大鼠已经表现出不同程度神经行为发育延迟、Morris水迷宫的空间学习记忆能 力降低,并表明低硒对大鼠脑EGFR/MAPK信号传导通路有一定影响,等等。为进 一步研究长期低硒低碘对仔代发育期大鼠的生长发育和神经行为发育影响的生 化分子机制,我们对仔三、四代发育期大鼠肝脏、全血、多脑区抗氧化酶系统及 脑中单胺类神经递质进行了测定。 方法 选取本课题组已成功建立的人工膳食低硒低碘喂养繁殖的SD大鼠动物模 型,仔三(F3)、四代(F4)新生4天(P4)和21天(P21)大鼠。模型分4组:(1)对 照组(Control组),硒水平0.1-0.3μ g/g,碘水平≥0.2μ g/g;(2)低硒组(Se- 组),硒水平0.02 μ g/g,碘水平≥0.2 μ g/g;(3)低碘组(I-组),硒水平0.1-
[Abstract]:Background and purpose Selenium is an essential trace element for human and animal life. Selenium deficiency causes a decrease in the activity of selenase, Oxygen free radical clearance blocked, endocrine disorders and a series of functional disorders, its impact on the nervous system is also More and more scholars pay attention to it. Selenium deficiency is closely related to iodine deficiency, which is often accompanied by low iodine deficiency. Selenium. After more than 20 years of universal iodine supplementation by the government, the typical cretinism caused by iodine deficiency has been basically brought under control. However, there are still many children with subclinical cretinism and mental retardation, so it is believed that selenium may be an important factor in subcretinism. The extraiodine factor. Previous studies on selenium and iodine deficiency focused on the primary generation and the offspring, and most of them were adult. For Long-term damage caused by simulated selenium and iodine deficiency disorders in populations living for generations in low selenium or combined iodine deficiency environments, especially It is a kind of intellectual damage to the children of offspring. We have worked with many scholars to establish the artificial diet with low selenium. The SD rat model was fed with iodine deficiency and lasted for nearly two years until the fourth generation. The third generation of this model Spatial learning and memory ability of Morris water maze with different degrees of neurobehavioral retardation in rats The effect of selenium deficiency on the EGFR/MAPK signal transduction pathway in the rat brain was decreased, and so on. To advance One-step study on the effects of long-term selenium and iodine deficiency on the growth and neurobehavioral development of rats in their offspring development The molecular mechanism of antioxidation enzymes in the liver, whole blood, multibrain regions of rats in the third and fourth generation of pups The monoamine neurotransmitters in the brain were determined. Method Selected SD rat model of artificial diet fed with low selenium and iodine, which has been successfully established by our research group P4) and P21 (21 days after birth) of the third generation of F _ 3 (F _ 4) and 21 days (P _ (4) (P _ (4) (P _ (4) and P _ (1). The model is divided into 4 groups: 1) Selenium level 0.1-0.3 渭 g / g, iodine level 鈮，

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