穿刺活检组织常规石蜡和常规冰冻HE制片的比较

发布时间：2018-05-29 09:44

本文选题：穿刺活检组织 + 石蜡切片　；参考：《山东医药》2014年41期

【摘要】：目的观察病理穿刺活检组织常规石蜡和常规冰冻制片HE染色效果的异同,提高穿刺活检组织的制片质量。方法取中山大学附属第一医院各手术科送检的病理穿刺活检组织标本(骨和骨髓、乳腺、脂肪)进行处理,每份送检标本均一分为二,一份常规固定脱水包埋切片;一份常规冰冻切片,两份标本切片均常规HE染色,镜下观察阅片并作比较。结果多数穿刺活检组织常规石蜡和常规冰冻切片制成的HE片结构完整,厚薄均匀,染色恰当,形态清晰,无断裂破碎现象,核浆分明,切片平整,定位准确。骨和骨髓穿刺活检组织不宜进行常规冰冻切片,也不能直接进行常规石蜡切片,必须经过脱钙液脱钙才能切片;含脂肪多的乳腺穿刺活检组织和脂肪组织做常规石蜡切片,脱水前必须用纯丙酮脱脂才能切片;而进行常规冰冻切片,组织切片不完整、折叠、破碎、脱落,严重影响制片质量和诊断的正确性。结论大多数病理穿刺活检组织都可以进行常规石蜡和常规冰冻切片,切片HE染色质量良好。
[Abstract]:Objective to observe the difference of HE staining effect between routine paraffin and frozen biopsy tissue and to improve the quality of biopsy tissue. Methods the biopsy specimens (bone and bone marrow, mammary gland, fat) of the first affiliated Hospital of Sun Yat-sen University were taken for treatment. Each specimen was divided into two parts, and a routine fixed dehydration embedded section was made. One routine frozen section and two specimens were stained with HE. Results the HE slices made from routine paraffin and frozen sections of most biopsy tissues were of complete structure, uniform thickness, proper staining, clear morphology, no fracture and fragmentation, clear nucleus and cytoplasm, flat sections and accurate orientation. Bone and bone marrow biopsy tissues are not suitable for routine frozen sections, nor can conventional paraffin sections be carried out directly. Decalcification of bone and bone marrow biopsy tissues must be done through decalcification solution before they can be sliced. Before dehydration, it is necessary to use pure acetone to defatted the sections, while the conventional frozen sections are not complete, folded, broken and shedded, which seriously affect the quality of film preparation and the correctness of diagnosis. Conclusion conventional paraffin and frozen sections can be performed in most pathological biopsy tissues, and the HE staining quality of the sections is good.
【作者单位】：中山大学附属第一医院;
【基金】：国家自然科学青年基金(81100470,81270750) 中山大学青年教师培育项目(12ykpy24) 广东省自然科学基金资助项目(S2012010009390)
【分类号】：R361.2

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