重组干扰素-α增强口蹄疫重组疫苗诱导滤泡辅助性T细胞的研究

发布时间：2018-06-02 07:26

本文选题：滤泡辅助性T细胞 + 干扰素-α　；参考：《免疫学杂志》2014年12期

【摘要】：目的通过观察重组干扰素-α(IFN-α)佐剂在口蹄疫(foot and mouth disease,FMD)重组疫苗免疫应答中对滤泡辅助性T细胞(follicular T helper cells,Tfh细胞)的免疫调节作用,为IFN-α佐剂免疫调节的新机制提供实验依据。方法 6周龄雌性Balb/c小鼠分为3组,实验组联合免疫表达猪IFN-α的重组腺病毒(r Ad5po IFN-α)和表达FMDV VP1的重组腺病毒(r Ad5VP1),另设2对照组,分别注射r Ad5VP1和空载体腺病毒(Advirus)。免疫后7 d,应用流式细胞术检测脾脏淋巴细胞中Tfh细胞(CD4+CXCR5+PD-1+)和生发中心(germinal center,GC)B细胞(B220+GL-7+)的增殖分化;real-time PCR技术检测Tfh细胞转录因子Bcl-6m RNA的表达水平;ELISA方法检测血清中细胞因子IL-21蛋白的表达水平。结果与单独免疫r Ad5VP1组相比,联合免疫r Ad5po IFN-α和r Ad5VP1能明显促进FMD重组腺病毒疫苗诱导小鼠Tfh细胞和GC B细胞的增殖分化;重组IFN-α能明显促进FMD重组腺病毒疫苗诱导小鼠Bcl-6 m RNA的表达,提高小鼠血清中IL-21蛋白的分泌水平。结论重组IFN-α能有效增强FMD疫苗诱导小鼠Tfh细胞免疫,为IFN-α佐剂免疫调节作用的新机制提供了理论依据。
[Abstract]:Objective to observe the immunomodulatory effect of recombinant interferon- 伪 (IFN- 伪) adjuvant on follicular helper T helper cells (Tfh) cells in the immune response to foot and mouth disease (FMD) and to provide experimental evidence for the new mechanism of IFN- 伪 adjuvant immunomodulation. Methods six week-old female Balb/c mice were divided into three groups. The experimental group was immunized with recombinant adenovirus expressing porcine IFN- 伪 (r Ad5po IFN- 伪) and the recombinant adenovirus expressing FMDV VP1 (rAd5VP1a), and two control groups were injected with r Ad5VP1 and empty vector adenovirus Advirusus respectively. On the 7th day after immunization, the expression of Tfh transcription factor Bcl-6m RNA was detected by flow cytometry (FCM) and germinal center-B cell (B220 GL-7) in splenic lymphocytes by flow cytometry and real-time PCR technique respectively and Elisa method was used to detect the expression level of Tfh transcription factor Bcl-6m RNA. Expression of cytokine IL-21 protein in serum. Results compared with r Ad5VP1 alone, combined immunization with r Ad5po IFN- 伪 and r Ad5VP1 could significantly promote the proliferation and differentiation of mouse Tfh cells and GC B cells induced by FMD recombinant adenovirus vaccine. Recombinant IFN- 伪 could significantly promote the expression of Bcl-6 m RNA in mice induced by FMD recombinant adenovirus vaccine and increase the secretion of IL-21 protein in serum of mice. Conclusion Recombinant IFN- 伪 can effectively enhance the immunogenicity of Tfh cells induced by FMD vaccine in mice, which provides a theoretical basis for the new mechanism of IFN- 伪 adjuvant immunomodulation.
【作者单位】：宁夏医科大学基础医学院病原生物学与免疫学系;宁夏医科大学检验学院临床免疫学检验系;
【基金】：国家自然科学基金(31001064,81260678,31360600) 宁夏自然科学基金(NZ13060) 宁夏教育厅高等学校科学研究项目(NGY2012065)
【分类号】：R392

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