小鼠PGRP-L分子的克

发布时间：2018-06-03 01:03

本文选题：小鼠PGRP-L + 克隆　；参考：《第一军医大学》2006年博士论文

【摘要】：1996年，Science发表了题为“The instructive role of innate immunity in the acquired immune response”的文章，，提出天然免疫的本质是识别病原微生物所特有的分子模式，除发挥第一线防御功能外，还指导获得性免疫以适当的方式应答以清除病原体的新观点。Janeway CA Jr等充实了这一理论，提出天然免疫通过模式识别作用启动获得性免疫应答并左右其应答类型。肽聚糖识别蛋白(peptidoglycan recognition protein，PGRP)家族分子是近年发现的一组重要的、从昆虫到哺乳动物都保守的、介导天然免疫的模式识别受体，可以识别细菌及其特有的细胞壁组分——肽聚糖(peptidoglycan，PGN)。哺乳动物PGRP是果蝇PGRP的同系物。目前已经克隆得到小鼠、大鼠、牛、骆驼、人等哺乳动物的短型PGRP(PGRP-S)和人PGRP家族的另外3种同源物——长型PGRP(PGRP-L)及2种中型PGRP(PGRP-Iα和PGRP-Iβ)。PGRP系统演化分析显示，PGRP家族里，PGRP-S是最古老而PGRP-L是最年轻的成员。哺乳动物PGRP-L形成一个与果蝇PGRP-L不相关的独立分支，提示哺乳动物PGRP-L不是源自果蝇PGRP-L、哺乳动物PGRP-S或PGRP-I，而来源于一个共同的昆虫PGRP-S祖先。研究表明，昆虫PGRP参与激活酚氧化酶原级联反应、激活Toll样受体、激活Imd途径和诱导吞噬作用。一些昆虫和哺乳类PGRP共有其中的酰胺酶活性，而哺乳类PGRP-S的抗菌活性为哺乳类所特有。哺乳动物PGRP-L在进化中的特殊性引起了我们的注意，而且其生物学功能尚属未知。基于mPGRP-L与hPGRP-L序列的高度同源性，小鼠无疑是进行PGRP-L结构、功能等方面研究的良好模型。本研究首先克隆了小鼠PGRP-L的
[Abstract]:In 1996, The instructive role of innate immunity in the acquired immune response published an article entitled "The instructive role of innate immunity in the acquired immune response", which proposed that the nature of innate immunity is a unique molecular pattern for the identification of pathogenic microorganisms, in addition to performing the first-line defense function. The new idea of guiding acquired immunity to respond in an appropriate way in order to eliminate pathogens enriches this theory by proposing that innate immunity initiates the acquired immune response by pattern recognition and determines its response type. Peptidoglycan recognition protein (PGRP) family is an important family discovered in recent years. It can recognize bacteria and its unique cell wall component peptidoglycan PGNN, which is conserved from insect to mammal and mediates innate immune pattern recognition receptor. Mammalian PGRP is a congener of Drosophila PGRP. So far, we've cloned mice, rats, cattle, camels, The phylogeny analysis of the short type PGRP-S of mammalian, the other three homologues of human PGRP family PGRPP-L) and the phylogeny of two medium-sized PGRP(PGRP-I 伪 and PGRP-I 尾. PGRP showed that PGRP-S is the oldest member and PGRP-L is the youngest member in the family. Mammalian PGRP-L formed an independent branch independent of Drosophila PGRP-L, suggesting that mammalian PGRP-L did not originate from Drosophila PGRP-S, mammalian PGRP-S or PGRP-Ibut from a common insect PGRP-S ancestor. It has been shown that insect PGRP is involved in the activation of phenoloxidase cascade reaction, activation of Toll like receptors, activation of Imd pathway and induction of phagocytosis. Some insect and mammalian PGRP share the activity of acylase, while the antibacterial activity of mammalian PGRP-S is unique to mammals. The particularity of mammalian PGRP-L in evolution has attracted our attention, and its biological function is unknown. Based on the high homology of mPGRP-L and hPGRP-L sequence, mouse is undoubtedly a good model to study the structure and function of PGRP-L. In this study, we first cloned mouse PGRP-L
【学位授予单位】：第一军医大学
【学位级别】：博士
【学位授予年份】：2006
【分类号】：R392

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