大鼠神经干细胞向多巴胺能神经元定向分化的实验研究

发布时间：2018-06-05 15:10

本文选题：神经干细胞 + 分化　；参考：《重庆医科大学》2006年硕士论文

【摘要】： 研究背景与目的神经干细胞(neural stem cell, NSC)是近年来神经科学领域的研究热点。NSC是具有多向分化潜能和自我更新的能力细胞,NSC的增殖分化为神经系统损伤和变性疾病的治疗带来了新希望。帕金森病(Parkinson disease,PD)是多巴胺能神经元减少或者消失导致的神经系统病变,增加DA能神经元是治疗该疾病的一种策略,通过对NSC进行体外诱导有望获得足量的有效多巴胺能神经元,为细胞移植治疗研究打下基础。本试验目的通过用IL-1β、AA、IL-1β联合AA诱导NSC分化生成多巴胺能神经元,为深入研究NSC体外分化条件,探讨诱导分化机制,提高DA能神经元分化效能奠定细胞学基础。方法分离培养新生(1日龄)SD大鼠中脑神经干细胞,通过传代培养扩增来检测自我更新能力;免疫学方法检测NSC特异性标志nestin蛋白表达和分化成为神经元、神经胶质细胞能力。对NSC克隆进行IL-1β、AA、IL-1β联合AA(抗坏血酸)诱导分化,通过免疫细胞学方法检测多巴胺能神经元标志物酪氨酸羟化酶(tyrosine tydroxylase,TH)表达。结果
[Abstract]:Research background and purpose Neural stem cell neural stem cell, NSC) is a research hotspot in the field of neuroscience in recent years. The proliferation and differentiation of neural stem cell neural stem cell, NSC), which has the potential of multiple differentiation and self-renewal, has brought new hope to the treatment of nervous system injury and degeneration. Parkinson disease (PD) is a neuropathy caused by the reduction or disappearance of dopaminergic neurons. Increasing DA neurons is a strategy for the treatment of the disease. It is hoped that sufficient effective dopaminergic neurons can be obtained by inducing NSC in vitro. To lay a foundation for the study of cell transplantation therapy. Objective to induce the differentiation of NSC into dopaminergic neurons by IL-1 尾 -AA-IL-1 尾 combined with AA, and to establish the cytological basis for further studying the differentiation conditions of NSC in vitro, exploring the mechanism of differentiation and enhancing the differentiation efficiency of DA neurons. Method The mesencephalic neural stem cells (NSCs) of 1-day-old SD rats were isolated and cultured, and the ability of self-renewal was detected by subculture and amplification. The expression and differentiation of nestin protein, a specific marker of NSC, into neurons and glial cells were detected by immunological methods. The NSC clone was induced by IL-1 尾 -AAAA-IL-1 尾 and ascorbic acid (ascorbic acid). The expression of tyrosine tydroxylasethase (THH), a dopaminergic neuron marker, was detected by immunocytology. Result
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R329

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