肝再生增强因子在新生和幼年大鼠重要器官中的表达差异研究

发布时间：2018-06-05 22:57

本文选题：肝再生增强因子 + 表达　；参考：《重庆医科大学》2006年硕士论文

【摘要】： 目的:观察肝再生增强因子在新生和幼年大鼠(分别为一天龄,一周龄,三周龄和五周龄四个时间点)的肝脏,脾脏,肌肉,胸腺四个器官中在核酸水平和蛋白水平的表达情况。为进一步探讨肝再生增强因子的免疫抑制作用与母婴传播途径引起的免疫耐受的关系提供实验依据。方法:雄性Wistar大鼠24只,按4个年龄阶段,分别是出生1天,7天,21天和35天分为四个组;每组6只。颈椎脱臼法处死大鼠;分别取其肝脏,脾脏,肌肉和胸腺四个器官分为两部分放于液氮中保存。其中一部份做RT-PCR检测ALRmRNA水平,另一部份做Western印迹法检测ALR蛋白水平。所有结果采用凝胶成像自动分析系统处理,所得的计量资料以均数±标准差(x±s)表示,采用单因素方差分析(One-Way ANOVA)和q检验,P0.05表示统计学差异,P0.01表示显著性差异。所有资料应用SPSS10.0统计软件进行分析。结果:(1)PCR结果显示:在未成年大鼠的肝脏,脾脏,肌肉,胸腺均有ALR-mRNA表达。在同一时间点上,ALR在肝脏中的表达量,比脾脏、肌肉和胸腺都高(P0.01),肌肉在所有检测器官中表达量最低。胸腺的表达量在前三个时间点上均明显高于脾脏,但在5周龄时表达量降低,与脾脏的表达量已无差异。ALR在同一器官中的表达量,随年龄的增加,ALR在各器官的表达量均逐渐降低。(2)Western blot结果显示:在未成年大
[Abstract]:Objective: to observe the liver, spleen and muscle of newborn and young rats at four time points of one day, one week, three weeks and five weeks old, respectively. Expression of nucleic acid and protein in four thymic organs. To further explore the relationship between the immunosuppressive effect of liver regeneration enhancer and the immune tolerance caused by mother-to-child transmission. Methods: Twenty-four male Wistar rats were divided into four groups according to four age stages, one day, seven days, 21 days and 35 days, with 6 rats in each group. The liver, spleen, muscle and thymus were divided into two parts and stored in liquid nitrogen. Some of them were detected for ALRmRNA by RT-PCR and the other for ALR by Western blotting. All the results were processed by the automatic analysis system of gel imaging. The measured data were expressed as mean 卤standard deviation (x 卤s). One-way ANOVA (single factor ANOVA) and Q test (P0.05) were used to indicate the statistical difference (P0.01). All data were analyzed by SPSS10.0 statistical software. Results ALR-mRNA expression was found in liver, spleen, muscle and thymus of juvenile rats. At the same time, the expression of ALR in liver was higher than that in spleen, muscle and thymus. The expression of thymus was significantly higher than that of spleen at the first three time points, but decreased at the age of 5 weeks, and there was no difference between thymus and spleen in the expression of ALR in the same organ. With the increase of age, the expression of ALR in all organs decreased gradually. The results of Western blot showed that:
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R341

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