太原市肠道病毒A71型基因特征分析

发布时间：2018-06-05 23:42

本文选题：肠道病毒A型 + 传播链　；参考：《中国疫苗和免疫》2014年03期

【摘要】：目的研究太原市手足口病(Hand,Foot and Mouth Disease,HFMD)患者中,肠道病毒A71型(Enterovirus Type A71,EVA71)的分子进化特征。方法采集太原市HFMD患者的咽拭子和粪便标本,采用荧光定量逆转录-聚合酶链反应(Reverse Transcription-Polymerase Chain Reaction,RT-PCR)方法检测EV核酸。然后随机选取11株EVA71阳性标本进行衣壳蛋白(Capsid Protein)VP1编码区基因扩增、核苷酸序列测定和分析,与其他48株各基因型和基因亚型的EV71代表株构建基因亲缘性关系树。结果太原市11株EVA71均属于C4基因亚型C4a进化分支,但11株EVA71在VP1编码区核苷酸和氨基酸的同源性分别为95.7%~100.0%和99.0%~100.0%。在亲缘进化树中显示,这些EVA71处在不同的簇中,表现为11株EVA71至少属于3条病毒传播链。结论太原市流行的EVA71属于C4基因亚型C4a进化分支,并且存在多个传播链。与中国其他地区流行的EVA71在基因亲缘关系和流行时间关系上都很接近,说明太原市EVA71不是独立进化的,而是与中国其他地区流行的EVA71在共同进化。
[Abstract]:Objective to study the molecular evolution characteristics of enterovirus A71 (Enterovirus Type A71, EVA71) in patients with Hand (Hand, Foot and Mouth Disease, HFMD). Methods the pharynx swabs and fecal specimens of the HFMD patients in Taiyuan were collected and the fluorescence quantitative reverse transcriptase chain reaction was used. RT-PCR method was used to detect EV nucleic acid. Then 11 EVA71 positive specimens were randomly selected to amplify the gene of capsid protein (Capsid Protein) VP1 coding region, nucleotide sequence determination and analysis, and construct genetic relationship tree with the other 48 genotypes and gene subtypes EV71 representative strains. The results of 11 EVA71 in Taiyuan belong to C4a of C4 subtype C4a. Evolutionary branches, but the homology of nucleotide and amino acids in the 11 EVA71 VP1 coding region is 95.7%~100.0% and 99.0%~100.0%. in the phylogenetic tree respectively. These EVA71 are in different clusters, showing that 11 strains of EVA71 belong to at least 3 virus transmission chains. Conclusion the prevalent EVA71 in Taiyuan belongs to the C4a evolutionary branch of the C4 gene subtype and is stored in the subtype of C4 gene. In multiple transmission chains, the EVA71, which is popular in other parts of China, is close to the relationship between genetic relationship and epidemic time. It shows that the Taiyuan EVA71 is not independent of evolution, but is coevolved with the popular EVA71 in other regions of China.
【作者单位】：太原市疾病预防控制中心;中国疾病预防控制中心病毒病预防控制所卫生部医学病毒学和病毒病重点实验室世界卫生组织西太平洋区脊髓灰质炎参比实验室;
【基金】：山西省卫生厅科学技术攻关项目(编号201302026) 国家科学技术艾滋病和病毒性肝炎等重大传染病防治重大专项——病毒性传染病病原谱流行规律及变异研究(编号2013ZX10004202) 国家重点基础研究发展计划(973计划:编号2011CB504902) 国家自然科学基金(编号30900063、30901259)
【分类号】：R373.11

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