新式线栓法建立大鼠局灶性脑缺血再灌注模型的建立

发布时间：2018-06-06 14:38

本文选题：线栓法 + 局灶性脑缺血再灌注　；参考：《中国民族民间医药》2014年02期

【摘要】：目的:在Zea Longa线栓法的基础上,对现有的改良线栓法进一步改进,为建立更加稳定的局灶性脑缺血再灌注模型提供参考。方法:30只雄性SD大鼠随机分Zea Longa线栓法组、改良线栓法组、新式线栓法组三组,每组10只。比较上述3组大鼠局灶性脑缺血再灌注模型的制作时间,及再灌注24h后大鼠的神经功能评分、脑梗死体积率及动物死亡率等指标,评价经过再改进后的新式线栓法制作的大鼠局灶性脑缺血再灌注模型的有效性。结果:Zea Longa线栓法组、改良线栓法组、新式线栓法组大鼠的神经功能评分、脑梗死体积率,模型成功率无明显差异(P0.01),但新式线栓法组的模型制作时间及大鼠死亡率明显低于Zea Longa线栓法组和改良线栓法组。结论:经过进一步改进的新式线栓法可以有效缩短模型制作时间,减少动物死亡率,优于Zea Longa线栓法组、改良线栓法组,使大脑中动脉阻塞再灌注模型的建立更加简易稳定。
[Abstract]:Aim: based on the method of Zea Longa thread embolization, the existing modified suture occlusion method was further improved to provide a reference for the establishment of a more stable model of focal cerebral ischemia-reperfusion. Methods 30 male Sprague-Dawley rats were randomly divided into three groups: Zea Longa suture group, modified thread embolization group and new thread embolization group with 10 rats in each group. The time of making focal cerebral ischemia-reperfusion model and the scores of neurological function, cerebral infarction volume rate and animal mortality were compared between the three groups after 24 hours of reperfusion. Objective: to evaluate the effectiveness of the modified new thread embolization method in the establishment of focal cerebral ischemia reperfusion model in rats. Results the neurological function score and cerebral infarct volume rate of rats in the groups of 1: Zea Longa suture method, modified thread embolus group and new thread embolus group were analyzed. There was no significant difference in the success rate of the model (P 0.01), but the time of making the model and the mortality of rats in the new method group were significantly lower than those in the Zea Longa group and the modified group. Conclusion: the new method can effectively shorten the time of making the model and reduce the mortality of animals. It is superior to the Zea Longa group and the modified method group, and makes the establishment of the reperfusion model of middle cerebral artery occlusion more simple and stable.
【作者单位】：贵阳中医学院基础医学院;
【基金】：贵州省科技厅联合基金项目:黔科合中药字[2011]LKZ7034号
【分类号】：R-332

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