二甲基亚硝胺诱发大鼠肝纤维化的形态学研究

发布时间：2018-06-08 22:22

本文选题：二甲基亚硝胺 + 肝纤维化　；参考：《延边大学》2005年硕士论文

【摘要】：目的:探讨二甲基亚硝胺诱发大鼠肝纤维化在两个不同时间段的病理形态学特征。方法:用1%二甲基亚硝胺腹腔注射4周诱发肝纤维化的动物模型,观察第4周和第7(染毒停止3周后)末大鼠肝/体重比、血清门冬氨酸转移酶、丙氨酸转移酶活性、总蛋白和总胆固醇含量的变化;观察肝组织的病理变化,检测肝组织内胶原纤维的面密度图。免疫组化利用ED1和α-SMA抗体观察肝组织内枯否氏细胞和星状细胞的表达。结果:第4周末模型组与对照组比较血清门冬氨酸转移酶、丙氨酸转移酶的活性和总胆固醇的含量明显升高,总蛋白含量明显下降;肝/体重比明显下降,肝组织内胶原纤维面密度明显升高,大部分(7/9)形成弥漫性肝硬化。免疫组化染色ED1+、α-SMA+细胞在增生的纤维间隔间大量弥漫分布,肝实质间散在分布。第7周末模型组与对照组比较除了血清总蛋白无明显变化外上述各项指标基本与第4周模型组相似,仍保持肝硬化的特点。结论:利用二甲基亚硝胺诱发的肝纤维化模型第4周末模型组大鼠肝功能明显受损伤,大部分表现为弥漫性肝硬化的形态学改变;染毒停止后3周末仍保持肝硬化的形态学特点。二甲基亚硝胺诱发的肝纤维化动物模型,适合进行肝纤维化的发生机制及抗肝纤维化药物疗效的研究。
[Abstract]:Objective: to investigate the pathomorphological characteristics of hepatic fibrosis induced by dimethylnitrosamine in rats at two different periods. Methods: the animal model of hepatic fibrosis induced by intraperitoneal injection of 1% dimethylnitrosamine for 4 weeks was established. The changes of liver / body weight ratio, serum aspartate transferase, alanine transferase activity, total protein and total cholesterol were observed at the end of week 4 and 7 (3 weeks after exposure), and the pathological changes of liver tissue were observed. The surface density of collagen fibers in liver tissue was measured. The expression of Kupffer cells and stellate cells in liver tissue was observed by immunohistochemical staining with ED1 and 伪 -SMA antibodies. Results: the serum aspartate transferase levels in the model group were compared with those in the control group at the end of the 4th week. The activity of alanine transferase and the content of total cholesterol increased obviously, the content of total protein decreased obviously, the ratio of liver to body weight decreased obviously, the density of collagen fibers in liver tissue increased obviously, most of them formed diffuse cirrhosis. Immunohistochemical staining showed that ED1, 伪 -SMA cells were distributed widely between proliferative fibrous septum and hepatic parenchyma. At the end of the 7th week, the above indexes in the model group were similar to those in the model group at the 4th week, except that there was no significant change in the serum total protein level between the model group and the control group. Conclusion: the hepatic fibrosis model induced by Dimethylnitrosamine at the end of the 4th week in the model group was obviously damaged, most of which were the morphological changes of diffuse cirrhosis. The morphologic features of liver cirrhosis remained at the end of 3 weeks after cessation of exposure. The animal model of hepatic fibrosis induced by Dimethylnitrosamine is suitable for studying the mechanism of hepatic fibrosis and the effect of anti-fibrosis drugs.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R363

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