FGR胎盘组织HGF、bFGF的表达及超微结构的研究

发布时间：2018-06-08 20:14

本文选题：胎儿生长受限 + 胎盘　；参考：《暨南大学》2006年硕士论文

【摘要】：研究目的： 1 观察FGR胎盘中HGF、bFGF的表达情况，分析胎盘绒毛间质微血管体视学变化，以探讨FGR的发病机理； 2 观察胎盘的绒毛滋养细胞和微血管内皮细胞的超微结构特征，探讨FGR的发病原因。材料和方法： 1 取人足月分娩正常妊娠胎盘组织(对照组n=15)，足月分娩FGR胎盘组织(FGR组n=15)，进行常规HE染色和免疫组织化学染色，并利用图像分析技术定量分析HGF和bFGF的表达强度，通过抗CD34标记血管内皮细胞显示血管的方法，进行胎盘绒毛间质内微血管体视学研究； 2 取人正常妊娠胎盘组织和FGR胎盘组织各三例，利用透射电镜技术进行观察。结果： 1 HGF和bFGF均表达于绒毛合体滋养层细胞、绒毛微血管内皮细胞和绒毛间质细胞，二者在胎盘中的表达强度以阳性单位PU值表示，在组间差别有统计学意义(P＜0.01)； 2 在实验中CD34几乎标记胎盘绒毛所有的血管，两组微血管长度密度在统计学上无明显差别(P＞0.05)，FGR组微血管体积密度小于对照组，统计学上有明显差别(P＜0.05)； 3 超微结构的观察发现FGR组胎盘组织出现下列改变包括：合体滋养层游离面微绒毛短小稀疏，顶部膨大，排列紊乱，部分区域见微绒毛密集增多，滋养细胞的粗面内质网扩张呈囊泡化，，线粒体肿胀呈空泡状变性，绒毛间质水肿，基底膜增厚、弯曲，见电子致密的球状颗粒沉积在基底膜或其邻近基质内。微血管内皮可见游离面伸出微绒毛样突起，自体胞质脱落，微血管壁可见钙化砂粒体。结论： 1 HGF和bFGF的表达在足月分娩FGR胎盘组织中较对照组明显增强，可能是
[Abstract]:Objective: 1 to observe the expression of HGF bFGF in placenta of FGR and analyze the stereological changes of stromal microvessels in placenta. To investigate the pathogenesis of FGR. 2 to observe the ultrastructural characteristics of villus trophoblast and microvascular endothelial cells in placenta. Materials and methods: 1 normal placenta tissues of human full-term delivery (control group) and full-term delivery group (FGR group) were collected for routine HE staining and immunohistochemical staining. The expression of HGF and bFGF was quantitatively analyzed by image analysis, and the vascular expression was displayed by anti-CD34 labeled vascular endothelial cells. Stereological study of intrastromal microvessels in placental chorionic villi was carried out. 2 Human normal placenta and FGR placenta were taken from 3 cases, and 3 cases from FGR placenta respectively. Results: 1HGF and bFGF were expressed in chorionic syncytiotrophoblast cells, chorionic microvascular endothelial cells and chorionic stromal cells. In the placenta, the expression intensity of CD34 was expressed as the positive unit pu, and the difference between the two groups was statistically significant (P < 0.01). 2 in the experiment, CD34 labeled almost all the blood vessels in placenta villi. There was no significant difference in microvessel length density between the two groups (P > 0.05). The volume density of microvessel in FGR group was lower than that in control group. (3) the observation of ultrastructure found that the following changes occurred in placental tissue of FGR group: the free surface microvilli of syncytiotrophoblast were short and sparse, the top of microvilli was dilated, and the arrangement of microvilli was disordered, and some areas showed dense increase of microvilli. The thicker endoplasmic reticulum of trophoblast was dilated with vesicle, mitochondria swelled with vacuolar degeneration, villous interstitial edema, thickening and bending of basement membrane, and electron dense globular particles deposited in basement membrane or its adjacent matrix. Microvillous protrusions were seen on the free surface of the microvascular endothelium and calcified sand bodies were found in the microvascular wall. Conclusion: 1the expression of HGF and bFGF in the placenta of full-term delivery FGR is significantly higher than that in the control group, and it may be that the expression of HGF and bFGF in the microvascular wall is significantly higher than that in the control group.
【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R361;R714.5

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