牛磺鹅去氧胆酸对小鼠免疫细胞凋亡的影响

发布时间：2018-06-10 21:07

本文选题：牛磺鹅去氧胆酸 + 免疫细胞　；参考：《内蒙古农业大学》2007年硕士论文

【摘要】： 本研究旨在阐明牛磺鹅去氧胆酸(TCDCA)对小鼠免疫细胞凋亡的影响及其影响机理。采用二苯胺法检测了给药后小鼠不同免疫细胞的凋亡百分率,结果显示:低剂量TCDCA能够显著抑制正常小鼠腹腔巨噬细胞、脾脏淋巴细胞和血液淋巴细胞的凋亡,但对胸腺细胞凋亡无显著影响;高剂量TCDCA能够显著抑制正常小鼠外周血淋巴细胞的凋亡,对脾脏淋巴细胞凋亡无显著影响,但能够显著促进正常小鼠腹腔巨噬细胞和胸腺细胞的凋亡。表明TCDCA对不同的免疫细胞的凋亡具有不同的影响,显示出其免疫调节作用。通过考察TCDCA对外周血淋巴细胞凋亡过程中Caspase依赖的内源性细胞凋亡途径的影响,探讨了其影响小鼠免疫细胞凋亡的机理。采用流式细胞仪检测了TCDCA对外周血液淋巴细胞凋亡早期磷脂酰丝氨酸外翻百分率的影响,证明低剂量和高剂量TCDCA均能够降低磷脂酰丝氨酸外翻百分率,用药后第4天,TCDCA对磷脂酰丝氨酸外翻百分率的抑制作用最显著;采用流式细胞仪检测了TCDCA对外周血淋巴细胞线粒体膜电位发生变化的百分率,证明低剂量TCDCA能够极显著的降低淋巴细胞线粒体膜势能变化百分率;采用分光光度法检测了TCDCA对外周血淋巴细胞凋亡过程中Caspase—3、Caspase—9蛋白酶的活化程度的影响,证明高、低剂量TCDCA均能极显著的抑制Caspase-9及Caspase-3蛋白酶活性。实验结果表明TCDCA对小鼠外周血淋巴细胞Caspase依赖的内源性细胞凋亡途径的不同时期、不同阶段均有显著的凋亡抑制作用。
[Abstract]:The aim of this study was to elucidate the effect of TCDCA on the apoptosis of immune cells in mice and the mechanism of its influence. The percentage of apoptosis in different immune cells of mice was detected by two aniline method. The results showed that the low dose TCDCA could significantly inhibit the peritoneal macrophages, spleen lymphocytes and blood lymphocytes in normal mice. Apoptosis has no significant effect on the apoptosis of thymus cells. High dose TCDCA can significantly inhibit apoptosis of peripheral blood lymphocytes in normal mice and have no significant influence on the apoptosis of spleen lymphocytes, but can significantly promote apoptosis of peritoneal macrophages and thymocytes in normal mice. It is indicated that TCDCA has no effect on the apoptosis of different immune cells. The same effect shows its immunomodulatory effect.
By investigating the effect of Caspase dependent endogenous apoptosis pathway on the apoptosis of peripheral blood lymphocyte in TCDCA, the mechanism of its influence on the apoptosis of immune cells in mice was investigated. The effect of TCDCA on the percentage of phosphatidyl serine ectropion in the early stage of apoptosis was detected by flow cytometry. The percentage of phosphatidylserine ectropion could be reduced by dose of TCDCA, and the inhibitory effect of TCDCA on the percentage of phosphatidylserine ectropion was the most significant after fourth days of drug use. The percentage of mitochondrial membrane potential changes in peripheral blood lymphocytes of TCDCA was detected by flow cytometry. It was proved that low dose TCDCA could significantly reduce lymphatic thinning. The percentage of mitochondrial membrane potential energy changes; the effect of the activation degree of Caspase - 3, Caspase - 9 protease in the apoptotic process of TCDCA peripheral blood lymphocytes was detected by spectrophotometric method. It was proved that high and low dose TCDCA could significantly inhibit the activity of Caspase-9 and Caspase-3 protease. The experimental results showed that TCDCA was in the peripheral blood of mice. In different stages of Caspase dependent endogenous apoptosis pathway, apoptosis of the Ba cells was significantly inhibited at different stages.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R392

【引证文献】