重组人肝再生增强因子的克

发布时间：2018-06-12 15:15

本文选题：人肝再生增强因子 + 基因表达　；参考：《浙江大学》2005年硕士论文

【摘要】：前言各种原因所致的急慢性肝功能衰竭严重威胁人类健康,其显著特点是短期内肝细胞大量严重坏死,病死率极高,目前发病机制尚不清楚,至今尚无有效的药物治疗方法。肝脏是再生能力很强的人体器官,有多种细胞因子参与调节这一过程,对肝再生调控因子的研究一直是一个热点,迄今已发现包括肝细胞生长因子(HGF)、转化生长因子-α(TGF-α)、表皮生长因子(EGF)、胰岛素等二十余种来自肝内外的参与调节肝再生的刺激因子和抑制因子,但它们均难以解释器官特异性的肝再生调控机制。1994年Hygiya等从初断乳大鼠肝组织中克隆得到的一种新型的促肝细胞增殖因子,命名为肝再生增强因子(ALR),肝再生增强因子是目前已知唯一对肝源性细胞有特异性增殖刺激活性的因子,且无种属特异性,实验证明该细胞因子能促进肝再生,并对各种急慢性肝损伤有保护作用,在肝损伤或部分肝切除后其表达量明显提高。本研究采用分子生物学技术,从正常人肝组织中获取人肝再生增强因子(hALR)阅读框全序列,结合pET28a(+)构建hALR原核表达体系,成功地从大肠杆菌中高效而稳定地表达出重组人肝再生增强因子蛋白质,并获高效纯化,为进一步进行蛋白质生物学功能鉴定和研制出一种新的抗肝损伤药物奠定基础。
[Abstract]:The acute and chronic liver failure caused by various causes is a serious threat to human health. Its remarkable characteristics are a large number of severe necrosis of liver cells in the short term and a high mortality rate. At present, the pathogenesis is not clear, so far there is no effective drug treatment. The liver is a human organ with strong regenerative ability. There are many cytokines involved in the regulation of this process. The research on the regulatory factors of liver regeneration has always been a hot topic. Up to now, more than 20 stimulating and inhibiting factors, including hepatocyte growth factor (HGF), transforming growth factor- 伪 (TGF- 伪), epidermal growth factor (EGFN), insulin and so on, have been found in and out of the liver to regulate liver regeneration. In 1994, Hygiya et al, a novel hepatocyte proliferative factor, was cloned from the liver tissue of the weaned rats, but it was difficult to explain the mechanism of organ specific regulation of liver regeneration. Liver regeneration enhancer is the only known factor that has specific proliferative stimulating activity to hepatogenic cells and has no species-specific. It has been proved that this cytokine can promote liver regeneration. It has protective effect on all kinds of acute and chronic liver injury, and its expression is obviously increased after liver injury or partial hepatectomy. In this study, the whole reading frame sequence of human augmenter of liver regeneration (hALR) was obtained from normal human liver tissue by molecular biology technique, and the prokaryotic expression system of hALR was constructed by pET28a (). The recombinant human liver regeneration enhancer protein was successfully expressed and purified from E. coli, which laid a foundation for further identification of protein biological function and development of a new anti-liver injury drug.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R346

【参考文献】